Mineral solution
Internal Solution-ID: S5001
This solution is used in 6 media and was first defined in medium J976.
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| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| EDTA | 3.7 | g | 3.7 | 12.661 | |
| FeSO4 x 7 H2O | 1.1 | g | 1.1 | 3.957 | |
| MnCl2 x 4 H2O | 98.4 | mg | 0.098 | - | |
| ZnSO4 x 7 H2O | 28.8 | mg | 0.029 | 0.1 | |
| CoCl2 x 6 H2O | 23.8 | mg | 0.024 | 0.183 | |
| CuCl2 x 2 H2O | 17.2 | mg | 0.017 | 0.101 | |
| Na2MoO4 x 2 H2O | 24.2 | mg | 0.024 | 0.1 | |
| H3BO3 | 31.0 | mg | 0.031 | 0.501 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 6.0 with KOH. | |||||
| 2 Comment: Consistent growth in serum tubes requires pretreatment of butyl rubber stoppers by boiling in 1% sodium sulfide solution (1 hr) followed by boiling in distilled H2O (1 hr, twice). | |||||