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Solution A

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Internal Solution-ID: S541

Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCl2 x 2 H2O 0.1 g 0.109 0.739
MgSO4 x 7 H2O 0.1 g 0.109 0.441
KH2PO4 0.5 g 0.543 3.994
K2HPO4 0.5 g 0.543 3.12
NaCl 1.0 g 1.087 18.6
Clarified rumen fluid 300.0 ml - -
Yeast extract
(OXOID) 		0.5 g 0.543 -
Peptone
(BD BACTO) 		0.5 g 0.543 -
(NH4)2SO4 0.5 g 0.543 4.113
Sodium resazurin
(0.1% w/v) 		0.5 ml 5.4e-4 0.002
L-Cysteine HCl x H2O 0.5 g 0.543 3.094
Distilled water 620.0 ml - -
275 TREPONEMA SUCCINIFACIENS MEDIUM