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Main sol. J1277

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Internal Solution-ID: S5421

Equipment needed: Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 18.00 g 17.699 302.86
MgCl2 x 2 H2O 1.00 g 0.983 7.492
MgSO4 x 7 H2O 1.00 g 0.983 3.989
KCl 0.30 g 0.295 3.957
Na2SO4 1.00 g 0.983 6.923
(NH4)2SO4 0.30 g 0.295 2.232
K2HPO4 0.05 g 0.049 0.282
KH2PO4 0.05 g 0.049 0.361
CaCl2 x 2 H2O 0.02 g 0.02 0.134
FeCl2 x 4 H2O 0.10 g 0.098 0.495
Iron(III) citrate 0.10 g 0.098 0.401
L-Cysteine HCl x H2O 0.50 g 0.492 -
MES 1.95 g 1.917 9.821
Resazurin 1.00 mg 9.8e-4 0.004
Trace element solution 1.00 ml - -
Se-Ni-W solution 1.00 ml - -
Distilled water 1000.00 ml - -
1 Mix components thoroughly, adjust pH to 6.3 and autoclave under a N2-CO2 (4:1, v/v) gas atmosphere. After cooling, add the following solutions from anaerobic stocks (autoclaved):
Yeast extract
(10%) 		5.00 ml - -
Sodium fumarate
(1.0 M) 		10.00 ml - -
2 Comment:
3 For JCM 39183, add 1.0 M glucose solution (final 10 mM), instead of sodium fumarate solution, and adjust pH to 5.5.
J1277 VULCANO HIGH SALT MEDIUM