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Main sol. J1283

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Internal Solution-ID: S5428

Equipment needed: Anaerobic workbench Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
HEPES 3.60 g 3.561 14.942
Na2SO4 3.24 g 3.205 22.562
K2HPO4 0.17 g 0.168 0.965
MgCl2 x 6 H2O 0.40 g 0.396 1.946
KCl 0.50 g 0.495 6.634
NH4Cl 0.30 g 0.297 5.548
NaHCO3 0.25 g 0.247 2.944
H3BO3 6.00 mg 0.006 0.096
Wolfe's mineral solution 1.00 ml - -
Sodium formate 0.68 g 0.673 9.89
Yeast extract 0.10 g 0.099 -
Resazurin 0.20 mg 2.0e-4 8.6e-4
Distilled water 1000.00 ml - -
1 Mix components thoroughly and adjust pH to 6.5. Distribute the medium into culture vessels (e.g., 25 ml in 120 ml-serum bottles) under a stream of a N2 gas, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solution (autoclaved) :
Na2S x 9 H2O
(5%) 		10.00 ml - -
J1283 SIUC-3 MEDIUM