Main sol. J1291
Internal Solution-ID: S5440
Equipment needed: Autoclave
Anaerobic workbench
| 1 Mix components thoroughly and adjust pH to 7.2 with NaOH. Bring to a boil, then cool down and distribute into culture vessels under a N2 gas stream, seal with butyl rubber stoppers, and autoclave. After cooling, aseptically and anaerobically add the following solution (autoclaved): | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 1.50 | g | 1.473 | 10.828 | |
| K2HPO4 | 2.90 | g | 2.849 | 16.355 | |
| Urea | 2.10 | g | 2.063 | 34.349 | |
| MOPS | 10.00 | g | 9.823 | 46.94 | |
| Yeast extract | 6.00 | g | 5.894 | - | |
| Trisodium citrate x 2 H2O | 2.90 | g | 2.849 | - | |
| L-Cysteine HCl x H2O | 0.25 | g | 0.246 | - | |
| Resazurin | 1.00 | mg | 9.8e-4 | 0.004 | |
| Distilled water | 1000.00 | ml | - | - | |
| GS2 salt solution | 10.00 | ml | - | - | |
|
Cellobiose
(0.2 M) |
8.00 | ml | - | - | |
Media with this solution
| J1291 | GS2 MEDIUM WITH CELLOBIOSE |