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Main sol. J1307

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Internal Solution-ID: S5464

Equipment needed: Anaerobic workbench Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 1.00 g 0.957 16.375
MgCl2 x 6 H2O 0.40 g 0.383 1.883
CaCl2 x 2 H2O 0.10 g 0.096 0.651
Na2SO4 4.00 g 3.828 26.948
NH4Cl 0.25 g 0.239 4.473
KH2PO4 0.20 g 0.191 1.406
KCl 0.50 g 0.478 6.418
Yeast extract 0.50 g 0.478 -
Sodium malate 0.89 g 0.852 4.783
Distilled water 1000.00 ml - -
1 Mix components and autoclave. Cool down under a N2 gas stream. Aseptically and anaerobically add per liter the following solutions from sterile anaerobic stocks :
Trace element solution 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
NaHCO3
(8%) 		30.00 ml - -
Vitamin solution 1.00 ml - -
Thiamine solution 1.00 ml - -
Vitamin B12 solution 0.05 g/L 1.00 ml - -
2 Adjust pH to 7.0-7.3. Aseptically and anaerobically distribute the medium into culture vessels (e.g., 10 ml medium in Balch tubes), fill the head space with a gas mixture of N2-CO2 (4:1, v/v), and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solution.
Na2S x 9 H2O
(5%) 		5.00 ml - -
L-Cysteine HCl x H2O
(5%) 		5.00 ml - -
J1307 PPLL MEDIUM