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J1307. PPLL MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.00 g 0.957 16.374
2 0.40 g 0.383 1.883
3 0.10 g 0.096 0.651
4 4.00 g 3.828 26.948
5 0.25 g 0.239 4.472
6 0.20 g 0.191 1.406
7 0.50 g 0.478 6.418
8 0.50 g 0.478 -
9 0.89 g 0.852 -
10 1000.00 ml 955.222 53022.8
11 Trace element solution 1.00 ml - -
12 Selenite-tungstate solution 1.00 ml - -
13
(8%)
30.00 ml 4.958 59.025
14 Vitamin solution 1.00 ml - -
15 Thiamine solution 1.00 ml - -
16 Vitamin B12 solution 1.00 ml - -
17
(5%)
5.00 ml - -
18
(5%)
5.00 ml - -
Instructions:
  1. Mix components and autoclave. Cool down under a N2 gas stream. Aseptically and anaerobically add per liter the following solutions from sterile anaerobic stocks : #11-16
  2. Adjust pH to 7.0-7.3. Aseptically and anaerobically distribute the medium into culture vessels (e.g., 10 ml medium in Balch tubes), fill the head space with a gas mixture of N2-CO2 (4:1, v/v), and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solution. #17-18
Solution occurs in 38 other media (first defined in medium J439 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(25%)
12.5 ml 3.963 108.679
2 2.1 g 2.1 7.553
3 30.0 mg 0.03 0.485
4 100.0 mg 0.1 -
5 190.0 mg 0.19 -
6 24.0 mg 0.024 -
7 2.0 mg 0.002 -
8 144.0 mg 0.144 -
9 36.0 mg 0.036 0.149
10 987.0 ml 985.23 54688.5
Solution occurs in 14 other media (first defined in medium J403 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 4 mg 0.04 0.292
2 1 mg 0.01 0.041
3 10 mg 0.1 0.812
4 5 mg 0.05 -
5 10 mg 0.1 -
6
(10 mM, pH 7.1)
100 ml - -
Solution occurs in 69 other media (first defined in medium J431 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.4 g 0.4 10.001
2 6.0 mg 0.006 -
3 8.0 mg 0.008 -
4 1000.0 ml 998.207 55408.8
Solution occurs in 10 other media (first defined in medium J403 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 10 mg 0.1 -
2
(25 mM, pH 3.4)
100 ml - -
Solution occurs in 18 other media (first defined in medium J403 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5 mg 0.05 0.032
2 100 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 958.075 53181.2
NaHCO3 4.958 59.025
Na2SO4 3.828 26.948
NaCl 0.957 16.374
Sodium malate 0.852 -
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