MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ
Back to all solutions

Main sol. 282a

Download this solution as

CSV JSON PDF

Internal Solution-ID: S549

Equipment needed: Filter Autoclave Gassing station Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.14 g 0.138 0.794
CaCl2 x 2 H2O 0.14 g 0.138 0.941
NH4Cl 0.25 g 0.247 4.618
MgSO4 x 7 H2O 3.40 g 3.36 13.631
MgCl2 x 6 H2O 4.10 g 4.051 19.928
KCl 0.33 g 0.326 4.374
NiCl2 x 6 H2O
(0.1% w/v) 		0.50 ml 4.9e-4 0.004
NaCl 18.00 g 17.787 304.356
Fe(NH4)2(SO4)2 x 6 H2O 0.01 g 0.01 -
Modified Wolin's mineral solution 10.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 4.9e-4 0.002
NaHCO3 1.00 g 0.988 11.763
Wolin's vitamin solution (10x) 1.00 ml - -
L-Cysteine HCl x H2O 0.50 g 0.494 2.813
Na2S x 9 H2O 0.50 g 0.494 2.057
Distilled water 1000.00 ml - -
1 Dissolve ingredients except bicarbonate, vitamins, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium into anoxic tubes to 30% of their volume under 80% H2 and 20% CO2 gas atmosphere and autoclave. Add vitamins (sterilized by filtration), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to use check pH of complete medium and adjust to 6.0, if necessary.
2 After inoculation add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure.
282a METHANOCALDOCOCCUS VILLOSUS MEDIUM