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Main sol. J1328

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Internal Solution-ID: S5490

Equipment needed: Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 18.00 g 17.613 301.378
MgCl2 x 6 H2O 2.60 g 2.544 12.514
CaCl2 x 2 H2O 0.15 g 0.147 0.998
NH4Cl 0.25 g 0.245 4.573
KH2PO4 0.20 g 0.196 1.438
KCl 0.50 g 0.489 6.563
NaNO3 0.85 g 0.832 9.785
Glucose 1.80 g 1.761 9.776
Yeast extract
(BD-Difco) 		1.00 g 0.978 -
Trace minerals
(see Medium No. 243) 		1.00 ml - -
Trace vitamins 1.00 ml - -
NaHCO3 2.52 g 2.466 29.352
Distilled water 1000.00 ml - -
1 Mix components, and adjust pH to 7.0. Dispense the medium into suitable culture vessels under a stream of N2-CO2 (80:20, v/v), seal with butyl rubber stoppers, and autoclave. Prior to inoculation, aseptically and anaerobically add per liter the following solutions (autoclaved and stored under a N2 atmosphere):
Na2S x 9 H2O
(5%) 		10.00 ml - -
L-Cysteine HCl x H2O
(5%) 		10.00 ml - -
J1328 PROLIXIBACTER MEDIUM