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Main sol. 465g

Internal Solution-ID: S5632

Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 3.5 g 3.465 24.411
KH2PO4 1.0 g 0.99 7.276
MgCl2 x 6 H2O 0.1 g 0.099 0.487
Trace element solution SL-4 1.0 ml - -
Distilled water 1000.0 ml - -
1 Prepare and autoclave medium. Add 5.5 g/l glycerol from a sterile solution. Add 645 mg/l cyanuric acid from a neutralized and filter sterilized stock solution and 10 ml vitamin solution (see medium 461) to the medium.
Glycerol 5.5 g/l 5.5 59.724
Cyanuric acid 645.0 mg/l 0.645 4.997
Vitamin solution 10.0 ml - -
2 Rehydrate and cultivate lyophilized cells in the complex medium recommended for the specific strain (e.g. medium 1, 220, 535 or 830). After this reactivation, cultivate on mineral medium with the appropriate carbon source.
465g MINERAL MEDIUM WITH CYANURIC ACID AS NITROGEN SOURCE