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Main sol. 480b

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Internal Solution-ID: S5635

Equipment needed: Hungate tubes Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.33 g 0.33 6.169
CaCl2 x 2 H2O 0.33 g 0.33 2.245
MgCl2 x 6 H2O 0.33 g 0.33 1.623
KCl 0.33 g 0.33 4.427
KH2PO4 0.33 g 0.33 2.425
Trace element solution SL-10 1.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 -
Sulfur
(powder) 		10.00 g 10 311.818
Distilled water 1000.00 ml - -
1 Add to the medium 0.5% casitone and 2.5% NaCl (marine source). Adjust pH to 5.7 with hydrochloric acid before sterilization. Sterilize the medium by heating for 1 h at 90 - 100°C on three successive days. Before use add 30.0 ml/l of 5% (w/v) sterile, anaerobic NaHCO3-solution, vitamins and sulfide. Final pH should be 6.5.
Casitone 0.50 % 5 -
NaCl 2.50 % 25 427.789
NaHCO3
(5% w/v) 		30.00 ml 1.5 17.856
Wolin's vitamin solution 10.00 ml - -
Na2S x 9 H2O 0.50 g 0.5 2.082
480b TTD-MEDIUM