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Main sol. 702

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Internal Solution-ID: S5647

Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.33 g 0.33 6.169
CaCl2 0.33 g 0.33 2.974
MgCl2 0.33 g 0.33 3.466
KCl 0.33 g 0.33 4.427
KH2PO4 0.33 g 0.33 2.425
NaCl 150.00 g 150 2566.74
NaHCO3 2.50 g 2.5 29.759
Na2S x 9 H2O 0.50 g 0.5 2.082
Glucose 5.00 g 5 27.753
Trace element solution SL-10
(SL 10) 		1.00 ml - -
Wolin's vitamin solution 10.00 ml - -
Resazurin 2.00 mg 0.002 0.009
Yeast extract 2.00 g 2 -
MgCl2 x 6 H2O 3.30 g 3.3 16.232
Cellulose , alternative
(microcrystalline) 		5.00 g 5 -
Cellobiose , alternative 5.00 g 5 14.607
Distilled water 990.00 ml - -
1 Prepare the medium without NaHCO3, Na2S, cellobiose or vitamins, boil and cool under a stream of nitrogen. Add the NaHCO3 and autoclave the medium. Na2S, cellobiose and vitamins are added from sterile, anaerobic stock solutions just before inoculating. When medium is prepared using microcrystalline cellulose instead of cellobiose as a substrate the microcrystalline cellulose must be added to the medium before autoclaving at the appropiate concentration. The final pH is 7.0.
702 HALOCELLA CELLULOLYTICA MEDIUM