DSMZ 702 . HALOCELLA CELLULOLYTICA MEDIUM

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Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.33 g 0.33 6.169
CaCl2 0.33 g 0.33 2.974
MgCl2 0.33 g 0.33 3.466
KCl 0.33 g 0.33 4.427
KH2PO4 0.33 g 0.33 2.425
NaCl 150.00 g 150 2566.74
NaHCO3 2.50 g 2.5 29.759
Na2S x 9 H2O 0.50 g 0.5 2.082
Glucose 5.00 g 5 27.753
Trace element solution SL-10
(SL 10)
1.00 ml - -
Wolin's vitamin solution 10.00 ml - -
Resazurin 2.00 mg 0.002 0.009
Yeast extract 2.00 g 2 -
MgCl2 x 6 H2O 3.30 g 3.3 16.232
Cellulose , alternative
(microcrystalline)
5.00 g 5 -
Cellobiose , alternative 5.00 g 5 14.607
Distilled water 990.00 ml - -
1 Prepare the medium without NaHCO3, Na2S, cellobiose or vitamins, boil and cool under a stream of nitrogen. Add the NaHCO3 and autoclave the medium. Na2S, cellobiose and vitamins are added from sterile, anaerobic stock solutions just before inoculating. When medium is prepared using microcrystalline cellulose instead of cellobiose as a substrate the microcrystalline cellulose must be added to the medium before autoclaving at the appropiate concentration. The final pH is 7.0.
Solution occurs in 263 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 150 2566.74
Glucose 5 27.753
Cellobiose 5 14.607
Cellulose 5 -
MgCl2 x 6 H2O 3.3 16.232
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain