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702. HALOCELLA CELLULOLYTICA MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Anaerobic workbench
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 mod. medium 591(medium 702) 1000.0 ml - -
2 2.5 g 2.5 29.76
3 0.5 g 0.5 2.082
4 Vitamin solution 10.0 ml - -
5 5.0 g 5 -
Instructions:
  1. Use medium 591 with the following modifications:
  2. Omit the peptone. Prepare the medium without NaHCO3, Na2S, cellobiose or vitamins, boil and cool under a stream of nitrogen. Add the NaHCO3 and autoclave the medium. Na2S, cellobiose and vitamins are added from sterile, anaerobic stock solutions just before inoculating. When medium is prepared using microcrystalline cellulose instead of cellobiose as a substrate the microcrystalline cellulose must be added to the medium before autoclaving at the appropiate concentration. The final pH is 7.0.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Equipment needed: Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.33 g 0.33 6.169
2 0.33 g 0.33 2.973
3 3.30 g 3.3 16.232
4 0.33 g 0.33 4.427
5 0.33 g 0.33 2.425
6 150.00 g 150 2566.63
9 5.00 g 5 27.754
10
(Bacto Proteose Peptone No.3)
5.00 g 5 -
11 Trace element solution SL-10 1.00 ml - -
13 2.00 mg 0.002 0.009
14 1000.00 ml 998.207 55408.8
15
(Oxoid)
2.00 g/l 2 -
Instructions:
  1. Prepare the medium without NaHCO3, Na2S, glucose and vitamins, boil and cool under a nitrogen:carbon dioxide (80:20 v/v) atmosphere. Glucose (filter-sterilized and stored under nitrogen), vitamins (filter sterilized and stored under nitrogen), and sodium sulphide (autoclaved under nitrogen) are added from sterile stock solutions. The pH should be pH 7.5.
  2. Look for further instructions at medium 591.
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Anaerobic workbench, Autoclave, Filter

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 1009.18 56017.7
NaCl 150 2566.63
Glucose 5 27.754
Microcrystalline cellulose or cellobiose 5 -
Peptone 5 -
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain

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