Chitin stock solution
Internal Solution-ID: S5654
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Chitin | 20 | g | 16.667 | - | |
|
HCl
(37 %) |
200 | ml | 74 | 2029.62 | |
| Distilled water | 1000 | ml | - | - | |
| 1 The chitin solution is prepared as follows: mix 20 g chitin (practical grade from crab shells) with 200 ml of 37% HCl (pre-cooled to 4°C) and stir for 1 hr at 4°C. Pour the suspension into 1 litre of distilled water (pre-cooled to 4°C) and filter through filter paper (Schleicher and Schüll Nr. 311853). Wash the residue five times with 500 ml distilled water and resuspend in 1 litre of distilled water. Neutralise the suspension with 10 ml of 5M KOH (final pH 6.5). Filter and wash with 3 litres of distilled water to remove KCl. | |||||
Media with this solution
| 766 | THERMOCOCCUS CHITINOPHAGUS MEDIUM |