Main sol. 1630c
Internal Solution-ID: S6278
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 1.00 | g | 1 | 17.112 | |
| MgCl2 x 6 H2O | 0.40 | g | 0.4 | 1.968 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| KH2PO4 | 0.20 | g | 0.2 | 1.47 | |
| KCl | 0.50 | g | 0.5 | 6.707 | |
| Na2SO4 | 0.05 | g | 0.05 | 0.352 | |
| Trace element solution (Nitrososphaera) | 1.00 | ml | - | - | |
| FeNaEDTA solution (7.5 mM) | 1.00 | ml | - | - | |
| NaHCO3 buffer (1M) | 2.00 | ml | - | - | |
| HEPES buffer solution | 10.00 | ml | - | - | |
| NH4Cl (1 M) | 2.00 | ml | - | - | |
| Sodiumpyruvate (1 M) | 1.00 | ml | - | - | |
| Double distilled water | 1000.00 | ml | - | - | |
| 1 Incubate all used glass ware and magnetic stirring rods in 0.1 M HCl and rinse 3x with double distilled water to remove residual detergents (always check visual for soap bubbles). | |||||
| 2 Autoclave the Basic-FWM and cool to room temperature. This medium should be free of any precipitations! | |||||
| 3 Aseptically, add per L of BASIC-FWM the following solutions from sterile stocks (do not store complemented FWM): - Trace element solution (Nitrososphaera) - FeNaEDTA solution - NaHCO3 solution - HEPES buffer solution - NH4Cl solution - Sodiumpyruvate solution | |||||
| 4 Adjust pH to 7.5. | |||||
Media with this solution
| 1630c | modified Nitrosospaera medium |