Main sol. 1845
Internal Solution-ID: S6447
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Basal salt stock solution (20x) | 50.0 | ml | - | - | |
| Trace element solution (Nitrososphaera) | 1.0 | ml | - | - | |
| FeNaEDTA solution (7.5 mM) | 1.0 | ml | - | - | |
| Selenite-tungstate solution | 1.0 | ml | - | - | |
| NH4Cl (1 M) | 0.5 | ml | - | - | |
| 1 Add 50 mL of 20x Basal salt stock solution and 4 g CaCO3 to 950 mL sterile double distilled water and mix all just before use. | |||||
| 2 Autoclave at 121 °C for 20 min. | |||||
| 3 Cool down to room temperature. | |||||
| 4 Add aseptic per liter of 1x basalt salt solution: 1 mL FeNaEDTA (7.5 mM), 1 mL trace elements solution, 1 mL Selenite-tungstate solution, 0.5 mL NH4Cl solution. | |||||
| 5 Adjust pH to 8.5 with NaOH. | |||||
Media with this solution
| 1845 | Nitrososphaera gargensis medium |