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DSMZ

DSMZ 1845 . Nitrososphaera gargensis medium

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1845 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Basal salt stock solution (20x) 50.0 ml - -
Trace element solution (Nitrososphaera) 1.0 ml - -
FeNaEDTA solution (7.5 mM) 1.0 ml - -
Selenite-tungstate solution 1.0 ml - -
NH4Cl (1 M) 0.5 ml - -
1 Add 50 mL of 20x Basal salt stock solution and 4 g CaCO3 to 950 mL sterile double distilled water and mix all just before use.
2 Autoclave at 121 °C for 20 min.
3 Cool down to room temperature.
4 Add aseptic per liter of 1x basalt salt solution: 1 mL FeNaEDTA (7.5 mM), 1 mL trace elements solution, 1 mL Selenite-tungstate solution, 0.5 mL NH4Cl solution.
5 Adjust pH to 8.5 with NaOH.

Basal salt stock solution (20x) #

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.00 g 1 7.348
KCl 1.50 g 1.5 20.121
Mg(SO4) x 7 H2O 7.00 g 7 -
NaCl 11.68 g 11.68 199.863
1 Prepare with double distilled water.
2 Autoclave at 121 °C for 20 min.
3 Cool down to room temperature.
4 Store in the dark at 4 °C.

Trace element solution (Nitrososphaera) #

1000
Solution occurs in 3 other media (first defined in Medium 1630c).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(12.5M) 		8 mL 3.646 100
H3BO3 30 mg 0.03 0.485
MnCl2 x 4 H2O 100 mg 0.1 0.505
CoCl2 x 6 H2O 190 mg 0.19 1.463
NiCl2 x 6 H2O 24 mg 0.024 0.185
CuCl2 x 2 H2O 2 mg 0.002 0.012
ZnSO4 x 7 H2O 144 mg 0.144 0.501
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Double distilled water 1000 ml - -
1 This solution contains no iron! Sterilise by autoclaving or filtration (0.2 µm) and store in the dark at 4 °C.

FeNaEDTA solution (7.5 mM) #

1000
Solution occurs in 3 other media (first defined in Medium 1630c).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeNa-EDTA 2753 mg 2.753 -
Double distilled water 1000 ml - -
1 Sterilise by filtration (0.2 µm) and store in the dark at 4 °C.

Selenite-tungstate solution #

1000
Solution occurs in 179 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

NH4Cl (1 M) #

50
Solution occurs in 3 other media (first defined in Medium 1630c).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 2.67 g 53.4 998.317
Double distilled water 50.00 ml - -
1 Dissolve 2.67 g ammonium chloride in 50 mL Double distilled water.
2 Filtrate (0.2 µm).

Metadata

Last modified: 19.09.24
Source: MediaDive: DSMZ
Taxonomic range:  Archaea
Medium type: Defined medium
Final pH: 8.5
Equipment needed: Autoclave, Filter
Reference: doi.org/10.1038/ismej.2016.192
Description:

Medium originally described for the cultivation of Nitrososphaera gargensis (Krümmel and Harms, 1982; Hatzenpichler et al., 2008) and modified for the cultivation of Nitrosotenuis aquarius and Nitrosocosmicus hydrocola.
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 0.584 9.993
Mg(SO4) x 7 H2O 0.35 -
KCl 0.075 1.006
KH2PO4 0.05 0.367
NH4Cl 0.027 0.499
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