Main sol. 350
Internal Solution-ID: S688
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| K2HPO4 | 2.21 | g | 2.21 | 12.688 | |
| KH2PO4 | 1.50 | g | 1.5 | 11.023 | |
| (NH4)2SO4 | 1.30 | g | 1.3 | 9.838 | |
| MgCl2 x 6 H2O | 0.10 | g | 0.1 | 0.492 | |
| CaCl2 x 2 H2O | 0.02 | g | 0.02 | 0.136 | |
| FeSO4 x 7 H2O | 1.25 | mg | 0.001 | 0.004 | |
| Yeast extract | 5.00 | g | 5 | - | |
|
Cellobiose
OR
Cellulose
(cellulose: MN 300) |
5.00 | g | 5 | 14.607 | |
| NaHCO3 | 0.80 | g | 0.8 | 9.523 | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.5 | 2.847 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 7.4 with 8 M NaOH. | |||||
| 2 Prepare the medium anaerobically under 100% nitrogen. Cellobiose, NaHCO3, and cysteine are sterilized separately by autoclaving under N2. | |||||
Media with this solution
| 350 | CELLULOMONAS FERMENTANS MEDIUM |