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DSMZ 350 . CELLULOMONAS FERMENTANS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 2.21 g 2.21 12.688
KH2PO4 1.50 g 1.5 11.023
(NH4)2SO4 1.30 g 1.3 9.838
MgCl2 x 6 H2O 0.10 g 0.1 0.492
CaCl2 x 2 H2O 0.02 g 0.02 0.136
FeSO4 x 7 H2O 1.25 mg 0.001 0.004
Yeast extract 5.00 g 5 -
Cellobiose OR Cellulose
(cellulose: MN 300)
5.00 g 5 14.607
NaHCO3 0.80 g 0.8 9.523
Resazurin 1.00 mg 1.0e-3 0.004
L-Cysteine HCl x H2O 0.50 g 0.5 2.847
Distilled water 1000.00 ml - -
1 Adjust pH to 7.4 with 8 M NaOH.
2 Prepare the medium anaerobically under 100% nitrogen. Cellobiose, NaHCO3, and cysteine are sterilized separately by autoclaving under N2.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.4
Equipment needed: Autoclave, Gassing station, Hungate tubes

Gas composition

  • 100 % N2

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Cellobiose 5 14.607
Cellulose 5 -
Yeast extract 5 -
K2HPO4 2.21 12.688
KH2PO4 1.5 11.023
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain

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