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Main sol. 358

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Internal Solution-ID: S697

Equipment needed: Water bath Gassing station Serum bottle
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 1.30 g 1.3 9.838
KH2PO4 0.28 g 0.28 2.058
MgSO4 x 7 H2O 0.25 g 0.25 1.014
CaCl2 x 2 H2O 0.07 g 0.07 0.476
FeCl3 x 6 H2O 0.02 g 0.02 0.074
Allen's trace element solution 10.00 ml - -
Yeast extract
(OXOID) 		1.00 g 1 -
Sulfur
(powder) 		5.00 g 5 155.909
Distilled water 1000.00 ml - -
1 Dissolve ingredients, except sulfur, bring medium to the boil, then cool to room temperature and adjust pH to 2.5 using 10 N H2SO4. Dispense medium under air atmosphere enriched with 1 - 10% CO2 in culture vessels suitable for anaerobic cultivation (e.g., 20 ml medium in 100 ml serum bottles) containing already the appropriate amount of sulfur. For sterilization sealed bottles with medium are heated in a boiling water bath for 2 - 3 h on each of 3 successive days.
2 Note: Inoculate with 5% (w/v) culture. Incubate without shaking.
358 ACIDIANUS MEDIUM (AEROBIC)