Artificial sea water
Internal Solution-ID: S827
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 24.7 | g | 24.7 | 422.656 | |
| KCl | 0.7 | g | 0.7 | 9.39 | |
| MgSO4 x 7 H2O | 6.3 | g | 6.3 | 25.56 | |
| MgCl2 x 6 H2O | 4.6 | g | 4.6 | 22.626 | |
| CaCl2 x 2 H2O | 1.3 | g | 1.3 | 8.843 | |
| NaHCO3 | 0.2 | g | 0.2 | 2.381 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 To prevent precipitation, CaCl2 and NaHCO3 are autoclaved each as separate stock solutions in 10 ml distilled water. | |||||
Media with this solution
| 405 | SP2 MEDIUM |