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Main sol. 431

Internal Solution-ID: S878

Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.00 g 1 7.348
NH4Cl 0.50 g 0.5 9.348
MgCl2 x 6 H2O 0.20 g 0.2 0.984
CaCl2 x 2 H2O 0.10 g 0.1 0.68
NaHCO3 3.00 g 3 35.711
NaCl 30.00 g 30 513.347
Vitamin solution (VA) 1.00 ml - -
Trace element solution (SLA) 1.00 ml - -
Na2S x 9 H2O 0.05 g 0.05 0.208
Na2S2O3 x 5 H2O 0.50 g 0.5 2.015
Na-malate 1.00 g 1 5.616
Distilled water 1000.00 ml - -
1 Adjust pH to 8.7, filter sterilize and add appropriate amount of Na2S solution.
431 ECTOTHIORHODOSPIRA VACUOLATA MEDIUM