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DSMZ

DSMZ 431 . ECTOTHIORHODOSPIRA VACUOLATA MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 431 #

ml
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.00 g 1 7.348
NH4Cl 0.50 g 0.5 9.348
MgCl2 x 6 H2O 0.20 g 0.2 0.984
CaCl2 x 2 H2O 0.10 g 0.1 0.68
NaHCO3 3.00 g 3 35.711
NaCl 30.00 g 30 513.347
Vitamin solution (VA) 1.00 ml - -
Trace element solution (SLA) 1.00 ml - -
Na2S x 9 H2O 0.05 g 0.05 0.208
Na2S2O3 x 5 H2O 0.50 g 0.5 2.015
Na-malate 1.00 g 1 5.616
Distilled water 1000.00 ml - -
1 Adjust pH to 8.7, filter sterilize and add appropriate amount of Na2S solution.

Vitamin solution (VA) #

100
Solution occurs in 3 other media (first defined in Medium 351).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 10 mg 0.1 0.409
Nicotinic acid amide 35 mg 0.35 2.866
Thiamine-HCl x 2 H2O 30 mg 0.3 0.804
p-Aminobenzoic acid 20 mg 0.2 1.458
Pyridoxal hydrochloride 10 mg 0.1 0.491
Calcium pantothenate 10 mg 0.1 0.21
Vitamin B12 5 mg 0.05 0.037
Distilled water 100 ml - -

Trace element solution (SLA) #

1000
Solution occurs in 4 other media (first defined in Medium 351).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeCl2 x 4 H2O 1.8 g 1.8 9.054
CoCl2 x 6 H2O 250.0 mg 0.25 1.925
NiCl2 x 6 H2O 10.0 mg 0.01 0.077
CuCl2 x 2 H2O 10.0 mg 0.01 0.059
MnCl2 x 4 H2O 70.0 mg 0.07 0.354
ZnCl2 100.0 mg 0.1 0.734
H3BO3 500.0 mg 0.5 8.085
Na2MoO4 x 2 H2O 30.0 mg 0.03 0.124
Na2SeO3 x 5 H2O 10.0 mg 0.01 0.038
Distilled water 1000.0 ml - -
1 Adjust pH to 2.0 - 3.0.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 8.7
Equipment needed: Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 30 513.347
NaHCO3 3 35.711
KH2PO4 1 7.348
Na-malate 1 5.616
NH4Cl 0.5 9.348
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain