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Main sol. 454

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Internal Solution-ID: S908

Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.20 g 0.209 1.537
NaCl 1.00 g 1.046 17.899
KCl 0.50 g 0.523 7.016
MgSO4 x 7 H2O 0.50 g 0.523 2.122
CaCl2 x 2 H2O 0.15 g 0.157 1.067
NH4Cl 0.25 g 0.262 4.889
Trace element solution SL-10 1.00 ml - -
Resazurin 1.00 mg 0.001 0.005
Na2SeO3 x 5 H2O
(0.26 g/ 1000 ml 10 mM NaOH) 		1.00 ml 0.003 0.01
Glycine 2.00 g 2.092 27.868
Distilled water 950.00 ml - -
1 pH 7.2 to 7.4
2 Before inoculation add per 100 ml of medium:
Biotin
(10 mg/l, autoclave for 10 minutes under N2) 		0.20 ml - -
NaHCO3
(84 g/l, autoclaved under CO2) 		3.00 ml - -
Na2S
(60 g/l, autoclaved under N2) 		1.00 ml - -
3 Prepare and distribute medium anaerobically by using the Hungate technique.
454 EBA MEDIUM