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Main sol. 492

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Internal Solution-ID: S998

Equipment needed: Gassing station Hungate tubes Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 954 ml - -
Solution B 20 ml - -
Solution C 10 ml - -
Solution D 1 ml - -
Solution E 20 ml - -
1 Sparge solution A with 100% N2 gas for at least 30 - 45 min to make it anoxic, then dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solution B is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions C and D are prepared under 100% N2 gas atmosphere and sterilized by filtration. Solution E is autoclaved separately under 100% N2 gas. Solutions B to E are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 6.8 - 7.0.
492 CLOSTRIDIUM METHYLPENTOSUM MEDIUM