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Solution A

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Internal Solution-ID: S999

Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.9 g 0.943 17.637
KH2PO4 0.9 g 0.943 6.932
NaCl 0.9 g 0.943 16.143
CaCl2 x 2 H2O 20.0 mg 0.021 0.143
MnCl2 x 4 H2O 20.0 mg 0.021 0.106
MgSO4 x 7 H2O 20.0 mg 0.021 0.085
CoCl2 x 6 H2O
(0.1% w/v) 		5.0 ml 0.005 0.04
FeSO4 x 7 H2O
(0.1% w/v in 0.1 N H2SO4) 		5.0 ml 0.005 0.019
ZnSO4 x 7 H2O
(0.1% w/v) 		2.0 ml 0.002 0.007
CuSO4 x H2O
(0.1% w/v) 		2.0 ml 0.002 0.013
Sodium resazurin
(0.1% w/v) 		0.5 ml 5.2e-4 0.002
Distilled water 940.0 ml - -
1 Adjust pH to 6.5 with KOH.
492 CLOSTRIDIUM METHYLPENTOSUM MEDIUM