Main sol. 12
Internal Solution-ID: S15
Equipment needed: Centrifuge
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| air-dried garden soil | 400 | g | 400 | - | |
| Tap water | 1000 | ml | - | - | |
| Agar | 15 | g | 15 | 44.599 | |
| 1 Sterilize 400.0 g of air-dried garden soil (with high content of organic matter) in 1000 ml tap water for one hour at 121°C. Allow it to sediment for a few hours at room temperature. Centrifuge the supernatant. Add 15.0 g agar per 1000 ml to the clear supernatant solution thus obtained. Adjust pH to 6.8 - 7.0 and sterilize. | |||||
Media with this solution
| 12 | SOIL EXTRACT MEDIUM |