Main sol. J401
Internal Solution-ID: S4129
Equipment needed: Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.20 | g | 15.385 | 113.051 | |
| NH4Cl | 0.25 | g | 19.231 | 359.521 | |
| NaCl | 1.00 | g | 76.923 | 1316.27 | |
| MgCl2 x 6 H2O | 0.40 | g | 30.769 | 151.347 | |
| KCl | 0.50 | g | 38.462 | 515.916 | |
| CaCl2 x 2 H2O | 0.15 | g | 11.539 | 78.485 | |
| Trace minerals | 1.00 | ml | - | - | |
| Trace vitamins | 10.00 | ml | - | - | |
|
Quinic acid
(5 mM) |
1.00 | ml | - | - | |
| Resazurin | 1.00 | mg | 0.077 | 0.336 | |
| NaHCO3 | 2.50 | g | 192.308 | 2289.19 | |
| 2,3-butanediol | 0.90 | g | 69.231 | 768.206 | |
| Na2S x 9 H2O | 0.36 | g | 27.692 | 115.297 | |
| Distilled water | 1.00 | ml | - | - | |
| 1 Adjust pH to 7.2. Prepare medium anaerobically under a N2-CO2 (4:1, v/v) gas mixture. | |||||
Media with this solution
| J401 | PROPIONIVIBRIO MEDIUM |