Main sol. J454
Internal Solution-ID: S4201
Equipment needed: Autoclave
Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 30.00 | g | 10000 | 171116 | |
| Na2CO3 | 2.76 | g | 920 | 8680.23 | |
| NaHCO3 | 24.00 | g | 8000 | 95230.2 | |
| KCl | 0.20 | g | 66.667 | 894.254 | |
| K2HPO4 | 0.20 | g | 66.667 | 382.755 | |
| MgCl2 x 6 H2O | 0.10 | g | 33.333 | 163.959 | |
| NH4Cl | 1.00 | g | 333.333 | 6231.69 | |
| Na2S x 9 H2O | 0.40 | g | 133.333 | 555.135 | |
| Yeast extract | 0.50 | g | 166.667 | - | |
| Glucose | 5.00 | g | 1666.67 | 9251.04 | |
| Trace vitamins | 2.00 | ml | - | - | |
| Trace minerals solution | 1.00 | ml | - | - | |
| Resazurin | 1.00 | mg | 0.333 | 1.454 | |
| 1 Dissolve components except Na2CO3, NaHCO3 and Na2S x 9H2O in 700 ml distilled water and autoclave under a N2 atmosphere in a bottle capped with a butyl rubber stopper. Separately dissolve Na2CO3 and NaHCO3 in 300 ml distilled water, filter-sterilize, and add to the medium aseptically and anaerobically. Adjust pH to 9.0 - 9.5, if necessary. Prior to inoculation, reduce the medium by the addition of a 5% Na2S x 9H2O solution autoclaved under a N2 atmosphere. | |||||
Media with this solution
| J454 | SPIROCHAETA CALIFORNIENSIS MEDIUM |