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Trace minerals solution

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Internal Solution-ID: S3903

This solution is used in 6 media and was first defined in medium J228.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.500 g 1.5 7.848
Fe(NH4)2(SO4)2 x 6 H2O 0.200 g 0.2 -
Na2SeO3 0.100 g 0.1 -
Na2MoO4 x 2 H2O 0.100 g 0.1 0.413
Na2WO4 x 2 H2O 1.000 g 1 3.032
MnSO4 x n H2O 0.100 g 0.1 -
ZnSO4 x 7 H2O 0.100 g 0.1 0.348
NiCl2 x 6 H2O 0.025 g 0.025 0.193
CuSO4 x 5 H2O 0.010 g 0.01 0.04
Distilled water 1000.000 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals.
2 Adjust final pH to 7.0.
J228 METHANOCOCCUS McC MEDIUM
J379 CARNOBACTERIUM PLEISTOCAENIUM MEDIUM
J454 SPIROCHAETA CALIFORNIENSIS MEDIUM
J1101 HALOLACTIBACILLUS 9G MEDIUM
J1112 MINERAL MEDIUM WITH GLUCOSE
J1113 ANAEROBIC MINERAL MEDIUM WITH YEAST EXTRACT