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JCM 1113 . ANAEROBIC MINERAL MEDIUM WITH YEAST EXTRACT

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1113 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 2.5 g 2.425 41.493
KCl 0.3 g 0.291 3.903
K2HPO4 0.3 g 0.291 1.671
MgSO4 x 7 H2O 0.1 g 0.097 0.394
NH4Cl 1.0 g 0.97 18.133
CaSO4 x 7 H2O 12.5 mg 0.012 0.089
Yeast extract 1.0 g 0.97 -
Fatty acid mixture 10.0 ml - -
Trace vitamins 2.0 ml - -
Trace minerals solution 1.0 ml - -
Resazurin 0.5 mg 4.8e-4 0.002
Distilled water 1000.0 ml - -
1 Mix components, adjust pH to 7.0 and autoclave under a N2 atmosphere in a bottle capped with a butyl rubber stopper. After cooling, add the following solution (autoclaved and stored under a N2 gas atmosphere):
Sodium Thiosulfate
(1.0 M) 		10.0 ml - -
Na2S x 9 H2O
(pH 7.0, 5%) 		8.0 ml - -

Fatty acid mixture #

20
Solution occurs in 5 other media (first defined in Medium J266).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Valeric acid 0.5 g 25 244.786
Isovaleric acid 0.5 g 25 244.786
alpha-Methylbutyric acid 0.5 g 25 244.786
Isobutyric acid 0.5 g 25 283.736
Distilled water 20.0 ml - -
1 Adjust pH to 7.5 with concentrated NaOH solution.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Trace minerals solution #

1000
Solution occurs in 5 other media (first defined in Medium J228).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.500 g 1.5 7.848
Fe(NH4)2(SO4)2 x 6 H2O 0.200 g 0.2 -
Na2SeO3 0.100 g 0.1 -
Na2MoO4 x 2 H2O 0.100 g 0.1 0.413
Na2WO4 x 2 H2O 1.000 g 1 3.032
MnSO4 x n H2O 0.100 g 0.1 -
ZnSO4 x 7 H2O 0.100 g 0.1 0.348
NiCl2 x 6 H2O 0.025 g 0.025 0.193
CuSO4 x 5 H2O 0.010 g 0.01 0.04
Distilled water 1000.000 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals.
2 Adjust final pH to 7.0.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 2.425 41.493
NH4Cl 0.97 18.133
Yeast extract 0.97 -
KCl 0.291 3.903
K2HPO4 0.291 1.671
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
oxygen obligate anaerobe 1 strain
temperature psychrophilic 1 strain