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Clarified rumen fluid

Internal Solution-ID: S610

This solution is used in 33 media and was first defined in medium 1310.

Equipment needed: Centrifuge Filter Autoclave
Instructions:
  1. Rumen fluid from cow or sheep (obtained from fistulated animals or abattoir refuse) is filtered through muslin, autoclaved at 121°C for 15 minand then centrifuged at 27,000 g for 20 min The supernatant is made anoxic by sparging with 100% N2 gas for 15 min dispensed under same gas atmosphere into anoxic serum vials to 30% of volume and then stored frozen at - 20°C.
78c CHOPPED MEAT MEDIUM FOR FERTIBACTERIUM SP.
119 METHANOBACTERIUM MEDIUM
119a METHANOBREVIBACTER MEDIUM
120 METHANOSARCINA MEDIUM
140 RUMINICLOSTRIDIUM MEDIUM
159 TREPONEMA BRYANTII MEDIUM
161 METHANOMICROBIUM MOBILE MEDIUM
201 DESULFONEMA LIMICOLA MEDIUM
212a TREPONEMA RUMINIS MEDIUM
212 SYNTHROPHOMONAS MEDIUM
213 SYNTHROPHOMONAS MEDIUM (SULFATE FREE)
275 TREPONEMA SUCCINIFACIENS MEDIUM
298 PELOBACTER PROPIONICUS MEDIUM (FRESHWATER)
300 RUMINOCOCCUS PASTEURII MEDIUM
320 CLOSTRIDIUM CELLULOVORANS MEDIUM
321 METHANOCORPUSCULUM AGGREGANS MEDIUM
322 METHANOSPHAERA (MCB-3) MEDIUM
334a METHANOTHRIX SOEHNGENII MEDIUM
336 OXOBACTER PFENNIGII MEDIUM
337 SYNTROPHOCOCCUS SUCROMUTANS MEDIUM
346 DESULFALLAS MEDIUM
347 SYNTROPHOMONAS SAPOVORANS MEDIUM
397 FERULATE MEDIUM
407 SYNTROPHOMONAS SD2 MEDIUM
523 CLOSTRIDIUM CHARTATABIDUM MEDIUM
666a CELLULOSILYTICUM MEDIUM
666 SUCCINICLASTICUM MEDIUM
704 BUTYRIVIBRIO SP. MEDIUM
734a METHANOBREVIBACTER CUTICULARIS MEDIUM
734 METHANOBREVIBACTER CURVATUS MEDIUM
912 TREPONEMA R8 MEDIUM
915 BLAUTIA MEDIUM
1310 BUTYRIVIBRIO M2 MEDIUM