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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: DL-Dithiothreitol

Your search received 31 results.

78c CHOPPED MEAT MEDIUM FOR FRETIBACTERIUM SP.

Compound Conc. [g/L] Conc. [mM]
Ground beef 586.166 -
Casein peptone 35.17 -
Na-formate 7.034 103.43
Na-fumarate 7.034 43.953
K2HPO4 5.862 33.654
Yeast extract 5.862 -
Ethanol 2.227 48.349
L-Cysteine HCl x H2O 0.586 3.337
DL-Dithiothreitol 0.352 2.279
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Solutions:
  • 50 ml Clarified rumen fluid
  • 20 ml Fatty acid mixture
  • 1 ml Wolin's vitamin solution (10x)
  • 5 ml Haemin solution
  • 2 ml Vitamin K1 solution
Gas phase:
  • 80 % N2
  • 20 % CO2

169b LEADBETTERA MEDIUM

Compound Conc. [g/L] Conc. [mM]
D-Glucose 3.988 22.136
Yeast extract 3.988 -
NaHCO3 0.997 11.868
L-Cysteine HCl x H2O 0.499 2.838
MgSO4 x 7 H2O 0.499 2.023
DL-Dithiothreitol 0.15 0.969
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Solutions:
  • 952 ml Solution A
  • 20 ml Solution B
  • 1 ml Solution D
  • 10 ml Solution E
  • 20 ml Solution C
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 1 ml Seven vitamins solution
Gas phase:
  • 100 % N2

212a TREPONEMA RECTALE MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na2SO4 2.778 19.556
Trehalose 1.884 5.504
Maltose x H2O 1.786 5.217
Acetic acid 1.763 29.355
Cellobiose 1.688 4.93
Sucrose 1.688 4.93
Na2CO3 1.488 14.04
Trypticase peptone 0.992 -
Ethanol 0.942 20.457
D-Glucose 0.794 4.405
D-Xylose 0.744 4.956
Propionic acid 0.591 7.977
Na-pyruvate 0.496 4.508
KH2PO4 0.496 3.645
NH4Cl 0.397 7.419
NaCl 0.397 6.79
DL-Dithiothreitol 0.397 2.572
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Solutions:
  • 917 ml Solution A
  • 30 ml Solution B
  • 4 ml Solution C
  • 4 ml Solution D
  • 30 ml Solution E
  • 1 ml Solution F
  • 2 ml Solution G
  • 10 ml Solution H
  • 10 ml Solution I
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 50 ml Clarified rumen fluid
  • 1 ml Vitamin K1 solution
  • 3.1 ml Volatile fatty acid mixture
  • 30 ml Sugar mix
  • 1 ml Seven vitamins solution
Gas phase:
  • 80 % N2
  • 20 % CO2

282b DESULFUROBACTERIUM HR11 MEDIUM

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 4.051 19.928
NaHCO3 0.988 11.763
DL-Dithiothreitol 0.988 6.404
Na2S2O3 x 5 H2O 0.988 3.981
KCl 0.326 4.374
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Solutions:
  • 10 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % H2
  • 20 % CO2

311a SPOROMUSA MEDIUM (H2/CO2)

Compound Conc. [g/L] Conc. [mM]
NaCl 2.239 38.31
Yeast extract 1.99 -
Casitone 1.99 -
Na2CO3 0.995 9.388
NH4Cl 0.498 9.301
MgSO4 x 7 H2O 0.498 2.019
K2HPO4 0.348 1.999
CaCl2 x 2 H2O 0.249 1.692
KH2PO4 0.229 1.682
DL-Dithiothreitol 0.149 0.967
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Solutions:
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % H2
  • 20 % CO2

322 METHANOSPHAERA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Methanol 7.704 240.443
NaHCO3 3.891 46.318
KH2PO4 2.724 20.015
Trypticase peptone 1.946 -
Yeast extract 1.946 -
NH4Cl 0.973 18.186
NaCl 0.584 9.987
K2HPO4 0.584 3.351
Na-formate 0.486 7.152
Na-acetate 0.486 5.929
DL-Dithiothreitol 0.486 3.152
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Solutions:
  • 100 ml Clarified rumen fluid
  • 10 ml Modified Wolin's mineral solution
  • 2 ml Wolin's vitamin solution (10x)
  • 3 ml FeSO4 x 7 H2O solution (0.1% w/v)
Gas phase:
  • 80 % H2
  • 20 % CO2

399a ARCHAEOGLOBUS NEPTUNIUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 17.946 307.087
MgCl2 x 6 H2O 3.988 19.616
Na-pyruvate 0.997 9.06
Na2SO3 0.598 4.746
Na2CO3 0.499 4.703
DL-Dithiothreitol 0.499 3.231
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Solutions:
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2

480 DESULFURELLA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Sulfur 9.98 311.195
Na-acetate 4.99 60.829
NaHCO3 2.495 29.7
NH4Cl 0.329 6.157
KCl 0.329 4.418
KH2PO4 0.329 2.42
CaCl2 x 2 H2O 0.329 2.24
MgCl2 x 6 H2O 0.329 1.62
DL-Dithiothreitol 0.299 1.94
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Solutions:
  • 1 ml Trace element solution SL-10
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2

503a SPOROMUSA TMAO3 MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na-DL-lactate 5 44.619
DL-Dithiothreitol 3 19.443
Na2CO3 1.5 14.153
NaCl 1 17.112
Casamino acids 1 -
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Solutions:
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 1 ml Seven vitamins solution
Gas phase:
  • 80 % N2
  • 20 % CO2

559 TMBS4 MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na-thiosulfate 3.321 21.003
NaCl 0.996 17.043
Syringic acid 0.598 -
KCl 0.498 6.68
MgCl2 x 6 H2O 0.398 1.96
NH4Cl 0.249 4.655
KH2PO4 0.199 1.464
CaCl2 x 2 H2O 0.149 1.016
HCl 0.002 0.068
FeCl2 x 4 H2O 0.001 0.008
NaOH 5.0e-4 0.012
Resazurin 5.0e-4 0.002
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 1.0e-4 5.0e-4
Pyridoxine hydrochloride 1.0e-4 4.8e-4
ZnCl2 7.0e-5 5.1e-4
Nicotinic acid 5.0e-5 4.0e-4
p-Aminobenzoic acid 5.0e-5 3.6e-4
(DL)-alpha-Lipoic acid 5.0e-5 2.4e-4
Thiamine HCl 5.0e-5 1.5e-4
Riboflavin 5.0e-5 1.3e-4
Vitamin B12 5.0e-5 3.7e-5
Calcium D-(+)-pantothenate 5.0e-5 -
Na2MoO4 x 2 H2O 3.6e-5 1.5e-4
NiCl2 x 6 H2O 2.4e-5 1.8e-4
Biotin 2.0e-5 8.2e-5
Folic acid 2.0e-5 4.5e-5
H3BO3 6.0e-6 9.7e-5
Na2WO4 x 2 H2O 4.0e-6 1.2e-5
Na2SeO3 x 5 H2O 3.0e-6 1.1e-5
CuCl2 x 2 H2O 2.0e-6 1.2e-5
NaHCO3 - -
Yeast extract - -
Distilled water - -
Dithiothreitol - -
Solutions:
  • 932 ml Solution A
  • 30 ml Solution B
  • 10 ml Solution C
  • 2 ml Solution D
  • 10 ml Solution E
  • 10 ml Solution F
  • 10 ml Solution G
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 10 ml Vitamin solution
  • 10 ml Syringate solution 6.0 %(w/v)
Gas phase:
  • 100 % N2
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