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169b. TREPONEMA AZOTONUTRICIUM MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2 Solution A 950 ml - -
3 Solution B 20 ml - -
4 Solution C 20 ml - -
5 Solution D 1 ml - -
6 Solution E 10 ml - -
Instructions:
  1. Dissolve ingredients of solution A (except cysteine) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add cysteine and adjust pH 7.2 with KOH, then distribute under the same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere, solution C is autoclaved under 100% N2 gas atmosphere and solutions D and E are sterilized by filtration under 100% N2 gas atmosphere. To complete the medium appropriate amounts of solutions B, C, D and E are added to the sterile solution A in the sequence as indicated. Final pH of the complete medium should be 7.5.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.04 g 0.042 0.286
2 0.50 g 0.525 2.131
3
(OXOID)
4.00 g 4.202 -
4 4.00 g 4.202 23.323
5 Trace element solution SL-10 1.00 ml - -
6 Selenite-tungstate solution 1.00 ml - -
7
(0.1% w/v)
0.50 ml 5.3e-4 0.002
8 0.50 g 0.525 2.99
9 950.00 ml 996.11 55292.4
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1 g 50 595.191
2 20 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(0.5 M)
16.04 ml - -
2
(0.5 M)
3.96 ml 910.8 6692.88
Instructions:
  1. Combine 16.04 ml of 0.5 M K2HPO4 with 3.96 ml of 0.5 M KH2PO4 stock solution to reach a pH of around 7.4.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(see mediuim 503)
1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.15 g 15 97.244
2 10.00 ml 998.207 55408.8
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 977.256 54245.8
KH2PO4 18.198 133.724
D-Glucose 3.988 22.134
Yeast extract 3.988 -
NaHCO3 0.999 11.892
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature psychrophilic 1 strain

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