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DSMZ

DSMZ 169 . TERETINEMA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 169 #

ml
Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 960 ml - -
Solution B 20 ml - -
Solution C 20 ml - -
1 Dissolve ingredients of solution A (except cysteine) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add cysteine and adjust pH 7.2 with KOH, then distribute under the same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere and solution C is autoclaved under 100% N2 gas atmosphere. To complete the medium appropriate amounts of solutions B and C are added to the sterile solution A in the sequence as indicated. Final pH of the complete medium should be 7.5.
2 For solid medium add 10.00 g/l agar (BD Bacto) to solution A.

Solution A #

960
Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCl2 x 2 H2O 0.04 g 0.042 0.283
MgSO4 x 7 H2O 0.50 g 0.521 2.113
Yeast extract
(OXOID) 		4.00 g 4.167 -
D-Glucose 2.00 g 2.083 11.564
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.2e-4 0.002
L-Cysteine HCl x H2O 0.50 g 0.521 2.965
Agar , for solid medium
optional 		10.00 g 10.417 -
Distilled water 960.00 ml - -

Solution B #

20
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 1 g 50 595.188
Distilled water 20 ml - -

Solution C #

20
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4
(0.5 M) 		16.04 ml 1.397 8.02
KH2PO4
(0.5 M) 		3.96 ml 0.269 1.98
1 Combine 16.04 ml of 0.5 M K2HPO4 with 3.96 ml of 0.5 M KH2PO4 stock solution to reach a pH of around 7.4.

Metadata

Last modified: 25.10.22
Source: DSMZ
Synonyms: TREPONEMA ZUELZERAE MEDIUM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar
optional 		10 -
Yeast extract 4 -
D-Glucose 2 11.101
NaHCO3 1 11.904
L-Cysteine HCl x H2O 0.5 2.847
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Cultivation metadata from 3 strains BacDive

phyla 1 2 strains
oxygen anaerobe 1 strain
oxygen facultative anaerobe 1 strain
temperature mesophilic 2 strains