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311a. SPOROMUSA MEDIUM (H2/CO2)

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.50 g 0.493 9.218
2 0.50 g 0.493 2.001
3 0.25 g 0.247 1.677
4 2.25 g 2.219 37.968
5
(0.1% w/v in 0.1 N H2SO4)
2.00 ml 0.002 0.007
6 Trace element solution SL-10 1.00 ml - -
7 Selenite-tungstate solution 1.00 ml - -
8 2.00 g 1.972 -
9 2.00 g 1.972 -
10
(0.1% w/v)
0.50 ml 4.9e-4 0.002
11 0.35 g 0.345 1.982
12 0.23 g 0.227 1.667
13 1.00 g 0.986 9.305
14 Vitamin solution 10.00 ml - -
15 0.15 g 0.148 0.959
16 1000.00 ml 984.425 54643.8
17 , might need strain adjustment 2.50 g/l - -
Instructions:
  1. Dissolve ingredients (except phosphates, carbonate, vitamins and dithiothreitol) and sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Add phosphates to the medium after autoclaving from sterile stock solutions prepared under 100% N2 gas and carbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins and dithiothreitol should be prepared under N2 gas and sterilized by filtration. Adjust pH of completed medium to pH 7.0.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Archaea
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 5
Strains with modifications: 5

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 996.228 55299
NaCl 2.219 37.968
Yeast extract 1.972 -
Casitone 1.972 -
Na2CO3 0.986 9.305
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Cultivation metadata from 5 strains BacDive

phyla 2 5 strains
oxygen anaerobe 5 strains
temperature mesophilic 5 strains

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