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311. SPOROMUSA MEDIUM (BETAINE)

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.50 g 0.493 9.218
2 0.50 g 0.493 2.001
3 0.25 g 0.247 1.677
4 2.25 g 2.219 37.968
5
(0.1% w/v in 0.1 N H2SO4)
2.00 ml 0.002 0.007
6 Trace element solution SL-10 1.00 ml - -
7 Selenite-tungstate solution 1.00 ml - -
8 2.00 g 1.972 -
9 2.00 g 1.972 -
10 6.70 g 6.607 48.887
11
(0.1% w/v)
0.50 ml 4.9e-4 0.002
12 0.35 g 0.345 1.982
13 0.23 g 0.227 1.667
14 1.00 g 0.986 9.305
15 Vitamin solution 10.00 ml - -
16 0.30 g 0.296 1.685
17 0.30 g 0.296 1.232
18 1000.00 ml 984.425 54643.8
Instructions:
  1. Dissolve ingredients (except phosphates, carbonate, vitamins, cysteine and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add phosphates, vitamins (sterilized by filtration), cysteine and sulfide to the medium after autoclaving from sterile stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of complete medium to pH 7.0, if necessary.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 8
Strains with modifications: 8

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 996.228 55299
Betaine x H2O 6.607 48.887
NaCl 2.219 37.968
Casitone 1.972 -
Yeast extract 1.972 -
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Cultivation metadata from 8 strains BacDive

phyla 1 8 strains
oxygen anaerobe 7 strains
temperature mesophilic 8 strains

Similar media

311b. SPOROMUSA MEDIUM (MARINE) 95 %
311a. SPOROMUSA MEDIUM (H2/CO2) 90 %
487. PEPTOCLOSTRIDIUM MEDIUM (BETAINE) 89 %
120a. METHANOSARCINA BARKERI MEDIUM 88 %
311c. SPOROMUSA ACIDOVORANS MEDIUM 86 %

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