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311c. SPOROMUSA ACIDOVORANS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.50 g 0.493 9.218
2 0.50 g 0.493 2.001
3 0.25 g 0.247 1.677
4 2.25 g 2.219 37.968
5
(0.1% w/v in 0.1 N H2SO4)
2.00 ml 0.002 0.007
6 Trace element solution SL-10 1.00 ml - -
7 Selenite-tungstate solution 1.00 ml - -
8 2.00 g 1.972 -
9 2.00 g 1.972 -
10
(0.1% w/v)
0.50 ml 4.9e-4 0.002
11 0.35 g 0.345 1.982
12 0.23 g 0.227 1.667
13 1.00 g 0.986 9.305
14 5.00 g 4.931 27.371
15 Vitamin solution 10.00 ml - -
16 0.30 g 0.296 1.685
17 0.30 g 0.296 1.232
18 1000.00 ml 984.425 54643.8
19 , might need strain adjustment 1.35 g/l - -
22 , might need strain adjustment 2.00 g/l - -
23 , might need strain adjustment 1.11 g/l - -
24 , might need strain adjustment 3.60 g/l - -
25 , optional, for pH adjustment
(5% w/v)
x - -
26 , might need strain adjustment 2.50 g/l - -
Instructions:
  1. Dissolve ingredients (except phosphates, carbonate, fructose, vitamins, cysteine and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add phosphates, fructose, vitamins, cysteine and sulfide to the medium from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Stock solutions of fructose and vitamins should be sterilized by filtration. Adjust pH of complete medium to pH 6.5 - 7.0, if necessary.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 17
Strains with modifications: 17

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 996.228 55299
D-Fructose 4.931 27.371
NaCl 2.219 37.968
Yeast extract 1.972 -
Casitone 1.972 -
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Cultivation metadata from 17 strains BacDive

halophily not 1 strain
halophily slightly 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 16 strains
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