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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

	Compound	Min. conc.	Max. conc.	Unit

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Current criteria: DL-Malic acid

Your search received 3 results.

Compound	Conc. [g/L]	Conc. [mM]
NaCl	18.943	324.147
MgCl₂ x 6 H₂O	8.973	44.137
DL-Malic acid	1.336	9.963
KNO₃	1.007	9.96
Na₂CO₃	0.997	9.407
KCl	0.499	6.687
KH₂PO₄	0.419	3.077
CaCl₂ x 2 H₂O	0.299	2.035
MgSO₄ x 7 H₂O	0.15	0.607
(NH₄)₂SO₄	0.1	0.755
Yeast extract	0.1	-
NaBr	0.05	0.485
L-Cysteine HCl x H₂O	0.05	0.284
SrCl2 x 6 H₂O	0.02	0.075
HCl	0.002	0.068
FeCl₂ x 4 H₂O	0.001	0.008
NaOH	5.0e-4	0.012
CoCl₂ x 6 H₂O	1.9e-4	0.001
MnCl₂ x 4 H₂O	1.0e-4	5.0e-4
Pyridoxine hydrochloride	1.0e-4	4.8e-4
ZnCl₂	7.0e-5	5.1e-4
Nicotinic acid	5.0e-5	4.0e-4
p-Aminobenzoic acid	5.0e-5	3.6e-4
(DL)-alpha-Lipoic acid	5.0e-5	2.4e-4
Calcium D-(+)-pantothenate	5.0e-5	2.1e-4
Thiamine HCl	5.0e-5	1.5e-4
Riboflavin	5.0e-5	1.3e-4
Na₂MoO₄ x 2 H₂O	3.6e-5	1.5e-4
NiCl₂ x 6 H₂O	2.4e-5	1.8e-4
Biotin	2.0e-5	8.2e-5
Folic acid	2.0e-5	4.5e-5
H₃BO₃	6.0e-6	9.7e-5
Na₂WO₄ x 2 H₂O	4.0e-6	1.2e-5
Na₂SeO₃ x 5 H₂O	3.0e-6	1.1e-5
CuCl₂ x 2 H₂O	2.0e-6	1.2e-5
Vitamin B₁₂	1.0e-6	7.4e-7
Distilled water	-	-
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Solutions:

1 ml Trace element solution SL-10
1 ml Selenite-tungstate solution
1 ml Wolin's vitamin solution (10x)

Gas phase:

80 % N2
20 % CO2

Compound	Conc. [g/L]	Conc. [mM]
NaCl	15	256.674
DL-Malic acid	4	29.831
BICINE buffer	1.632	10
K₂HPO₄	1	5.741
NH₄Cl	0.52	9.721
MgSO₄ x 7 H₂O	0.2	0.811
CaCl₂ x 2 H₂O	0.075	0.51
EDTA	0.005	0.018
FeCl₂ x 4 H₂O	0.002	0.008
Thiamine	1.0e-3	0.004
CoCl₂ x 6 H₂O	1.9e-4	0.001
Na₂MoO₄ x 2 H₂O	1.9e-4	7.8e-4
MnCl₂ x 4 H₂O	1.0e-4	5.1e-4
ZnCl₂	7.0e-5	5.1e-4
VOSO₄ x 2 H₂O	3.0e-5	1.5e-4
Biotin	3.0e-5	1.2e-4
NiCl₂ x 6 H₂O	2.5e-5	1.9e-4
Vitamin B₁₂	2.0e-5	1.5e-5
CuCl₂ x 2 H₂O	1.7e-5	1.0e-4
H₃BO₃	6.0e-6	9.7e-5
Na₂WO₄ x 2 H₂O	2.0e-6	6.1e-6
NaHSeO₃	2.0e-6	-
Deionized water	-	-
Distilled water	-	-
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Solutions:

1 ml Trace elements

Compound	Conc. [g/L]	Conc. [mM]
Na₂CO₃	9	84.915
KCl	5.4	72.435
K₂HPO₄	0.3	1.722
CaCl₂ x 2 H₂O	0.29	1.973
NaNO₃	0.27	3.177
Bacto peptone	0.25	-
Sodium formate	0.2	2.941
Sodium acetate	0.2	2.438
Sodium pyruvate	0.2	1.818
Fumaric acid	0.2	1.723
DL-Malic acid	0.2	1.492
Trisodium citrate x 2 H₂O	0.2	-
Disodium succinate x 6 H₂O	0.2	-
MgCl₂ x 6 H₂O	0.1	0.492
Yeast extract	0.05	-
Sodium lactate	-	-
Distilled water	-	-
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981 GEOTHERMOBACTER MEDIUM

1147 RV5 MEDIUM

J1264 JCM MEDIUM No. 1264

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