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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.
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Current criteria: Gellan Gum
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1291 CHTHONOMONAS MEDIUM
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| (NH4)2SO4 | 4 | 30.271 |
| MgCl2 x 6 H2O | 1 | 4.919 |
| Gellan Gum | 0.5 | - |
| K2HPO4 | 0.45 | 2.584 |
| Yeast extract | 0.01 | - |
| Na2-EDTA x 2 H2O | 0.005 | 0.015 |
| FeCl2 x 4 H2O | 0.002 | 0.008 |
| Pyridoxine hydrochloride | 3.0e-4 | 0.001 |
| Nicotinic acid | 2.0e-4 | 0.002 |
| Thiamine-HCl x 2 H2O | 2.0e-4 | 5.4e-4 |
| CoCl2 x 6 H2O | 1.9e-4 | 0.001 |
| MnCl2 x 4 H2O | 1.0e-4 | 5.1e-4 |
| Calcium pantothenate | 1.0e-4 | 2.1e-4 |
| Vitamin B12 | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 8.0e-5 | 5.8e-4 |
| ZnCl2 | 7.0e-5 | 5.1e-4 |
| Na2MoO4 x 2 H2O | 3.6e-5 | 1.5e-4 |
| NiCl2 x 6 H2O | 2.4e-5 | 1.9e-4 |
| D-(+)-biotin | 2.0e-5 | 8.2e-5 |
| H3BO3 | 6.0e-6 | 9.7e-5 |
| CuCl2 x 2 H2O | 2.0e-6 | 1.2e-5 |
| Distilled water | - | - |
| CaCl2 x 2 H2O | - | - |
Solutions:
- 1 ml Trace element solution SL-11
- 1 ml Seven vitamins solution
J1035 VXG GELLAN
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Gellan Gum | 190.476 | - |
| MES | 46.429 | 237.803 |
| Xylan | 11.905 | - |
| FeCl2 x 4 H2O | 0.036 | 0.18 |
| NaOH | 0.012 | 0.298 |
| CoCl2 x 6 H2O | 0.005 | 0.035 |
| MnCl2 x 4 H2O | 0.002 | - |
| ZnCl2 | 0.002 | 0.012 |
| Na2MoO4 x 2 H2O | 8.6e-4 | 0.004 |
| NiCl2 x 6 H2O | 5.7e-4 | 0.004 |
| H3BO3 | 1.4e-4 | 0.002 |
| Na2WO4 x 2 H2O | 9.5e-5 | 2.9e-4 |
| Na2SeO3 x 5 H2O | 7.1e-5 | 2.7e-4 |
| CuCl2 x 2 H2O | 4.8e-5 | 2.8e-4 |
| Distilled water | - | - |
| (NH4)2HPO4 | - | - |
| MgCl2 x 6 H2O | - | - |
| MgSO4 x 7 H2O | - | - |
| HCl | - | - |
| CaCl2 x 2 H2O | - | - |
Solutions:
- 1 ml Selenite-tungstate solution
- 1 ml Trace element solution SL-10