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Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
MES | 1.95 | g | 46.429 | 237.803 | |
MgSO4 x 7 H2O
(20 mM) |
10.00 | ml | - | - | |
CaCl2 x 2 H2O
(30 mM) |
10.00 | ml | - | - | |
(NH4)2HPO4
(20 mM) |
10.00 | ml | - | - | |
MgCl2 x 6 H2O
(1 M) |
10.00 | ml | - | - | |
Selenite-tungstate solution | 1.00 | ml | - | - | |
Trace element solution SL-10 | 1.00 | ml | - | - | |
Xylan | 0.50 | g | 11.905 | - | |
Gellan Gum | 8.00 | g | 190.476 | - | |
1 Adjust pH to 5.5 |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaOH | 0.5 | g | 0.5 | 12.501 |
Na2SeO3 x 5 H2O | 3.0 | mg | 0.003 | 0.011 |
Na2WO4 x 2 H2O | 4.0 | mg | 0.004 | 0.012 |
Distilled water | 1000.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
HCl
(25%, 7.7 M) |
10.0 | ml | 2.5 | 68.568 | |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
Distilled water | 990.0 | ml | - | - | |
1 Dissolve FeCl2 x 4H2O first in HCl. Then dilute in distilled water and add the remaining salts. |
Last modified: | 22.02.22 |
Source: | JCM |
Medium type: | Complex medium |
Final pH: | 5.5 |
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