JCM 1035 . VXG GELLAN

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
MES 1.95 g 46.429 237.803
MgSO4 x 7 H2O
(20 mM)
10.00 ml - -
CaCl2 x 2 H2O
(30 mM)
10.00 ml - -
(NH4)2HPO4
(20 mM)
10.00 ml - -
MgCl2 x 6 H2O
(1 M)
10.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Trace element solution SL-10 1.00 ml - -
Xylan 0.50 g 11.905 -
Gellan Gum 8.00 g 190.476 -
1 Adjust pH to 5.5
Solution occurs in 179 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -
Solution occurs in 20 other media (first defined in Medium J433).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%, 7.7 M)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 Dissolve FeCl2 x 4H2O first in HCl. Then dilute in distilled water and add the remaining salts.

Metadata

Last modified: 22.02.22
Source: JCM
Medium type: Complex medium
Final pH: 5.5
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Gellan Gum 190.476 -
MES 46.429 237.803
Xylan 11.905 -
FeCl2 x 4 H2O 0.036 0.18
NaOH 0.012 0.298
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