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Current criteria: L-Methionine

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1494 . OMIZ-PAT Medium (modified)

Compound Conc. [g/L] Conc. [mM]
(NH4)6Mo7O24 x 4 H2O 12.4 -
Yeast extract 3 -
Fe SO4 x 7 H2O 2.78 10
D-Arabinose 2 13.322
L-Rhamnose 2 12.183
L-Fucose 2 12.183
D-Mannose 2 11.102
D-Glucose 2 11.101
D-Trehalose 2 5.843
D-Sucrose 2 5.843
NH4Cl 1.6 29.912
Glutathione 1.537 5
Ethanol 1 21.706
NaHCO3 1 11.904
Ascorbic acid 1 5.678
KCl 0.968 12.985
D-Fructose 0.8 4.441
D-Mannitol 0.8 4.392
D-glucuronic acid 0.8 4.121
D-galacturonic acid 0.8 4.121
D-Maltose 0.8 2.337
L-Glutamine 0.68 4.653
L-Histidine 0.62 3.996
NaVO3 0.61 5.003
D 0.56 4.866
Pyruvic acid 0.55 6.246
L-Serine 0.525 4.996
Fumaric acid 0.5 4.308
HCl 0.365 10
L-Cysteine HCl 0.35 2.221
Formic acid 0.3 6.518
L-Glutamic acid 0.3 2.039
ZnSO4 x 7 H2O 0.287 0.998
MgSO4 x 7 H2O 0.25 1.014
DL-carnitine 0.2 1.241
Citric acid 0.2 1.041
N-Acetylglucosamine 0.2 0.904
L-Lysine HCl 0.182 -
L-Arginine 0.175 1.005
L-Ornithine HCl 0.17 -
L-Phenylalanine 0.165 0.999
CaCl2 x H2O 0.15 1.352
L-Asparagine 0.15 1.135
L-Methionine 0.15 1.005
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Solutions:
  • 200 ml Solution H
  • 8 ml Solution I
  • 2 ml Solution J
  • 40 ml Solution K
  • 2 ml Solution L
  • 200 ml Trace elements III
  • 10 ml Solution A
  • 10 ml Solution B
  • 10 ml Solution C
  • 10 ml Solution D
  • 10 ml Solution E
  • 10 ml Solution F
  • 10 ml Solution G
  • 2 ml Trace elements I
  • 2 ml Trace elements II
  • 200 ml Supplement 1
  • 2 ml Supplement 2
  • 2 ml Supplement 3
  • 2 ml Supplement 5
  • 200 ml Supplement 4

1615 Basic mineral medium

Compound Conc. [g/L] Conc. [mM]
K2HPO4 2.264 13
KH2PO4 0.953 7
Sodium acetate 0.82 10
Na-aspartate 0.354 2
MgSO4 x 7 H2O 0.246 1
CaCl2 x 2 H2O 0.05 0.34
L-Methionine 0.007 0.05
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Solutions:
  • 1000 ml 20 mM K-P-Buffer
  • 1 ml Trace element solution
  • 0.5 ml Vitamine mixture
  • 100 ml Acidic thiamine / Ca pantothenate solution
  • 100 ml Basic vitamin solution
  • 100 ml Neutral folic acid / riboflavin solution
  • 100 ml Neutral B12 solution 0.005 g/L

1700 TRICHLOROBACTER AMMONIFICANS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na2CO3 0.998 9.416
Na-acetate 0.798 9.733
KH2PO4 0.798 5.867
K2HPO4 0.798 4.584
KNO3 0.499 4.936
MgSO4 x 7 H2O 0.15 0.607
Na-thioglycolate 0.1 0.875
Ascorbic acid 0.1 0.567
CaCl2 x 2 H2O 0.02 0.136
L-Methionine 0.008 0.054
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Solutions:
  • 1 ml Trace element solution SL-10
Gas phase:
  • 80 % N2
  • 20 % CO2

1825 MDM

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 5 35.221
L-Proline 2 17.372
NaH2PO4 x H2O 2 16.67
L-Cysteine 2 16.507
alpha-D-Glucose 2 11.102
NaCl 0.9 15.4
KH2PO4 0.9 6.614
L-Leucine 0.4 3.049
L-Valine 0.3 2.561
L-Isoleucine 0.3 2.287
L-Methionine 0.2 1.34
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Solutions:
  • 100 ml Salt mix 10x
  • 10 ml Trace salt mix 100x
  • 3 ml Iron stock solution 2.0 g/L
  • 5 ml Vitamine mixture
  • 10 ml Glucose stock 20%
  • 200 ml Amino acids mix 5x