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78a CHOPPED MEAT MEDIUM FOR TREPONEMA SP.

Compound Conc. [g/L] Conc. [mM]
Ground beef 473.934 -
Casitone 28.436 -
Agar 14.218 42.274
K2HPO4 4.739 27.21
Yeast extract 4.739 -
L-Glutamine 0.664 4.54
L-Histidine 0.569 3.666
L-Serine 0.474 4.51
L-Cysteine HCl 0.474 3.007
Ethanol 0.18 3.909
Vitamin K3 0.047 -
Hemin 0.005 0.007
Resazurin 9.5e-4 0.004
p-Aminobenzoic acid 2.4e-4 0.002
alpha-lipoic acid 2.4e-4 0.001
Pantothenic acid 2.4e-4 0.001
Thiamine-HCl x 2 H2O 2.4e-4 6.3e-4
Riboflavin 2.4e-4 6.3e-4
Vitamin B12 2.4e-4 1.7e-4
Nicotine amide 1.2e-4 9.7e-4
Nicotinic acid 1.2e-4 9.6e-4
Biotin 9.5e-5 3.9e-4
Folic acid 9.5e-5 2.1e-4
Pyridoxamine hydrochloride 4.7e-5 2.3e-4
NaOH - -
Water - -
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Solutions:
  • 10 ml Haemin solution
  • 10 ml Vitamin K3 solution
  • 10 ml Vitamin K1 solution
  • 5 ml Vitamin solution
  • 50 ml Amino acid solution (filter sterilized)

78d MEDIUM FOR TREPONEMA PARVUM

Compound Conc. [g/L] Conc. [mM]
Ground beef 500 -
Casitone 30 -
Agar 15 44.599
K2HPO4 5 28.707
Glutathione 1.4 4.556
Na-pyruvate 0.5 4.544
L-Cysteine HCl 0.5 3.172
N-Acetylglucosamine 0.5 2.26
Ethanol 0.19 4.124
Casamino acids 0.16 -
Yeast extract 0.16 -
Tryptone peptone 0.16 -
Ascorbic acid 0.1 0.568
L-Glutamine 0.07 0.479
L-Histidin 0.06 0.387
L-Serin 0.05 0.476
Vitamin K3 0.05 -
Resazurin 1.0e-3 0.004
Hemin 5.0e-4 7.7e-4
p-Aminobenzoic acid 2.5e-4 0.002
alpha-lipoic acid 2.5e-4 0.001
Pantothenic acid 2.5e-4 0.001
Thiamine-HCl x 2 H2O 2.5e-4 6.7e-4
Riboflavin 2.5e-4 6.6e-4
Vitamin B12 2.5e-4 1.8e-4
Nicotine amide 1.3e-4 0.001
Nicotinic acid 1.3e-4 0.001
Biotin 1.0e-4 4.1e-4
Folic acid 1.0e-4 2.3e-4
Pyridoxamine hydrochloride 5.0e-5 2.4e-4
Calf serum - -
Vitamin K1 - -
KCl - -
Water - -
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Solutions:
  • 10 ml Haemin solution
  • 10 ml Vitamin K3 solution
  • 10 ml Vitamin K1 solution
  • 5 ml Vitamin solution
  • 5 ml Amino acid solution
  • 5 ml Peptone mixture
  • 5 ml Hemin solution
  • 5 ml Ascorbic acid solution 2%(w/v)
  • 5 ml N-acetylglucosamin- and pyruvate solution
  • 10 ml Glutathione solution 14%(w/v)
  • 10 ml Sugar solution
Gas phase:
  • 80 % N2
  • 20 % CO2

110 CHOPPED MEAT MEDIUM WITH CARBOHYDRATES

Solutions:
  • 10 ml Haemin solution
  • 10 ml Vitamin K3 solution
  • 10 ml Vitamin K1 solution
Gas phase:
  • 100 % N2

129 POTATO DEXTROSE AGAR

Compound Conc. [g/L] Conc. [mM]
Potato 200 -
Glucose 20 111.012
Agar 15 44.599
Water - -
Solutions:
  • 1000 ml Potato infusion

209 EUBACTERIUM LENTUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
Ground beef 500 -
Casitone 30 -
Agar 15 44.599
K2HPO4 5 28.707
Arginine 5 28.703
Yeast extract 5 -
L-Cysteine HCl 0.5 3.172
Ethanol 0.19 4.124
Vitamin K3 0.05 -
Hemin 0.005 0.008
Resazurin 1.0e-3 0.004
NaOH - -
Vitamin K1 - -
Distilled water - -
Water - -
Solutions:
  • 10 ml Haemin solution
  • 10 ml Vitamin K3 solution
  • 10 ml Vitamin K1 solution

277 CHERRY AGAR (CBS formula)

Compound Conc. [g/L] Conc. [mM]
Pulp of sour stone cherries 40 -
Agar 20 59.465
Distilled water - -
Water - -
Solutions:
  • 200 ml Cherry extract

413 BTU MEDIUM

Compound Conc. [g/L] Conc. [mM]
Ground beef 500 -
Casitone 30 -
Agar 15 44.599
K2HPO4 5 28.707
Yeast extract 5 -
Na-formiate 1.8 26.468
Na-fumarate 1.8 11.248
L-Cysteine HCl 0.5 3.172
Ethanol 0.19 4.124
Vitamin K3 0.05 -
Hemin 0.005 0.008
Resazurin 1.0e-3 0.004
Water - -
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Solutions:
  • 10 ml Haemin solution
  • 10 ml Vitamin K3 solution
  • 10 ml Vitamin K1 solution
  • 30 ml Formiate/Fumarate Solution

457a MINERAL MEDIUM WITH 2-CHLOROBENZOATE

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 2.416 17.018
KH2PO4 1.505 11.059
2-Chlorobenzoic acid 0.775 4.951
(NH4)2SO4 0.495 3.746
MgSO4 x 7 H2O 0.198 0.803
CaCl2 x 2 H2O 0.05 0.337
EDTA 0.005 0.017
FeSO4 x 7 H2O 0.002 0.007
H3BO3 3.0e-4 0.005
CoCl2 x 6 H2O 2.0e-4 0.002
ZnSO4 x 7 H2O 9.9e-5 3.4e-4
MnCl2 x 4 H2O 3.0e-5 1.5e-4
Na2MoO4 x 2 H2O 3.0e-5 1.2e-4
NiCl2 x 6 H2O 2.0e-5 1.5e-4
CuCl2 x 2 H2O 9.9e-6 5.8e-5
Water - -
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Solutions:
  • 10 ml Trace element solution SL-4
  • 10 ml 2-chlorobenzoic acid solution
  • 100 ml Trace element solution SL-6

457c MEDIUM WITH CHLOROACRYLIC ACID

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 2.44 17.188
KH2PO4 1.52 11.17
(NH4)2SO4 0.5 3.784
MgSO4 x 7 H2O 0.2 0.811
3-Chloroacrylic acid 0.08 0.751
CaCl2 x 2 H2O 0.05 0.34
EDTA 0.005 0.017
FeSO4 x 7 H2O 0.002 0.007
H3BO3 3.0e-4 0.005
CoCl2 x 6 H2O 2.0e-4 0.002
ZnSO4 x 7 H2O 1.0e-4 3.5e-4
MnCl2 x 4 H2O 3.0e-5 1.5e-4
Na2MoO4 x 2 H2O 3.0e-5 1.2e-4
NiCl2 x 6 H2O 2.0e-5 1.5e-4
CuCl2 x 2 H2O 1.0e-5 5.9e-5
Distilled water - -
Water - -
Solutions:
  • 10 ml Trace element solution SL-4
  • 20 ml 3-chloroacrylic acid solution
  • 100 ml Trace element solution SL-6

810 SKIM MILK/GLUCOSE AGAR

Compound Conc. [g/L] Conc. [mM]
Skim milk 40 -
Glucose 20 111.012
Agar 20 59.466
Water - -
Solutions:
  • 500 ml Solution A
  • 500 ml Solution B
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