1083
1083 .
ACIDULIPROFUNDUM MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1083 #
ml
Equipment needed: Hungate tubes
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 30.00 | g | 29.97 | 512.834 | |
| MgSO4 x 7 H2O | 3.50 | g | 3.497 | 14.186 | |
| MgCl2 x 6 H2O | 2.75 | g | 2.747 | 13.513 | |
| CaCl2 x 2 H2O | 0.38 | g | 0.38 | 2.582 | |
| KCl | 0.33 | g | 0.33 | 4.422 | |
| NaBr | 0.05 | g | 0.05 | 0.485 | |
| (NH4)2SO4 | 0.10 | g | 0.1 | 0.756 | |
| KH2PO4 | 0.28 | g | 0.28 | 2.055 | |
| Wolfe's mineral elixir | 1.00 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| Na3-citrate | 2.94 | g | 2.937 | 11.381 | |
| Yeast extract | 1.00 | g | 0.999 | - | |
|
Tryptone
(BD Bacto) |
1.00 | g | 0.999 | - | |
|
Sulfur
(powdered) |
10.00 | g | 9.99 | 311.506 | |
| Na2S x 9 H2O | 0.50 | g | 0.5 | 2.08 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except sulfur and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials containing already the appropriate amount of powdered sulfur and autoclave for 60 min at 105°C. Prior to inoculation add sulfide from a sterile anoxic stock solution prepared under 100% N2 gas and adjust pH of complete medium to 4.5. | |||||
Solution occurs in 21
other media (first defined in Medium 792).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MgSO4 x 7 H2O | 30.00 | g | 30 | 121.716 | |
| MnSO4 x H2O | 5.00 | g | 5 | 29.583 | |
| NaCl | 10.00 | g | 10 | 171.116 | |
| FeSO4 x 7 H2O | 1.00 | g | 1 | 3.597 | |
| CoCl2 x 6 H2O | 1.80 | g | 1.8 | 13.863 | |
| CaCl2 x 2 H2O | 1.00 | g | 1 | 6.802 | |
| ZnSO4 x 7 H2O | 1.80 | g | 1.8 | 6.26 | |
| CuSO4 x 5 H2O | 0.10 | g | 0.1 | 0.401 | |
| AlK(SO4)2 x 12 H2O | 0.18 | g | 0.18 | 0.379 | |
| H3BO3 | 0.10 | g | 0.1 | 1.617 | |
| Na2MoO4 x 2 H2O | 0.10 | g | 0.1 | 0.413 | |
| (NH4)2Ni(SO4)2 x 6 H2O | 2.80 | g | 2.8 | 7.089 | |
| Na2WO4 x 2 H2O | 0.10 | g | 0.1 | 0.303 | |
| Na2SeO4 | 0.10 | g | 0.1 | 0.529 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts. | |||||
Metadata
| Last modified: | 24.07.23 |
| Source: | DSMZ |
| Taxonomic range: | Archaea |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 4.5 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 80 % N2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 2 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Sulfur | 9.99 | 311.506 |
| Na3-citrate | 2.937 | 11.381 |
| MgCl2 x 6 H2O | 2.747 | 13.513 |
| Tryptone | 0.999 | - |
| Yeast extract | 0.999 | - |
| Show more | ||
