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Wolfe's mineral elixir

Internal Solution-ID: S1605

This solution is used in 22 mediaand was first defined in medium 792.

Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 30.00 g 30 121.716
MnSO4 x H2O 5.00 g 5 29.583
NaCl 10.00 g 10 171.116
FeSO4 x 7 H2O 1.00 g 1 3.597
CoCl2 x 6 H2O 1.80 g 1.8 13.863
CaCl2 x 2 H2O 1.00 g 1 6.802
ZnSO4 x 7 H2O 1.80 g 1.8 6.26
CuSO4 x 5 H2O 0.10 g 0.1 0.401
AlK(SO4)2 x 12 H2O 0.18 g 0.18 0.379
H3BO3 0.10 g 0.1 1.617
Na2MoO4 x 2 H2O 0.10 g 0.1 0.413
(NH4)2Ni(SO4)2 x 6 H2O 2.80 g 2.8 7.089
Na2WO4 x 2 H2O 0.10 g 0.1 0.303
Na2SeO4 0.10 g 0.1 0.529
Distilled water 1000.00 ml - -
1 First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts.
792a THERMOVIBRIO AMMONIFICANS MEDIUM
792 PYROLOBUS FUMARII MEDIUM
792c NAUTILIA NITRATIREDUCENS MEDIUM
792b CAMINIBACTER MEDIATLANTICUS MEDIUM
1028 THERMINCOLA MEDIUM (CO)
1030 SMITHELLA MEDIUM
1040 MARIDESULFOVIBRIO MEDIUM
1041 DESULFUROMUSA FERRIREDUCENS MEDIUM
1042 VULCANIBACILLUS MEDIUM
1074 DESULFOGLAEBA MEDIUM
1083 ACIDULIPROFUNDUM MEDIUM
1092 THERMOSULFIDIBACTER MEDIUM
1098 ARCHAEOGLOBUS INFECTUS MEDIUM
1115 CONGREGIBACTER (SYPHC) MEDIUM
1188b MARISPIROCHAETA MEDIUM
1188 SPIROCHAETA CELLOBIOSIPHILA MEDIUM
1267b GALLIONELLA (ES) MEDIUM
1267a MARIPROFUNDUS (ES) MEDIUM
1459 THERMOHALOBACTER MEDIUM
1475 METALLIBACTERIUM MEDIUM
1526b KIRITIMATIELLA (TYG) MEDIUM
1665 DESULFAMPLUS MEDIUM