Wolfe's mineral elixir
Internal Solution-ID: S1605
This solution is used in 22 media and was first defined in medium 792.
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| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MgSO4 x 7 H2O | 30.00 | g | 30 | 121.716 | |
| MnSO4 x H2O | 5.00 | g | 5 | 29.583 | |
| NaCl | 10.00 | g | 10 | 171.116 | |
| FeSO4 x 7 H2O | 1.00 | g | 1 | 3.597 | |
| CoCl2 x 6 H2O | 1.80 | g | 1.8 | 13.863 | |
| CaCl2 x 2 H2O | 1.00 | g | 1 | 6.802 | |
| ZnSO4 x 7 H2O | 1.80 | g | 1.8 | 6.26 | |
| CuSO4 x 5 H2O | 0.10 | g | 0.1 | 0.401 | |
| AlK(SO4)2 x 12 H2O | 0.18 | g | 0.18 | 0.379 | |
| H3BO3 | 0.10 | g | 0.1 | 1.617 | |
| Na2MoO4 x 2 H2O | 0.10 | g | 0.1 | 0.413 | |
| (NH4)2Ni(SO4)2 x 6 H2O | 2.80 | g | 2.8 | 7.089 | |
| Na2WO4 x 2 H2O | 0.10 | g | 0.1 | 0.303 | |
| Na2SeO4 | 0.10 | g | 0.1 | 0.529 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts. | |||||
Media with this solution
| 792 | PYROLOBUS FUMARII MEDIUM |
| 792c | NAUTILIA NITRATIREDUCENS MEDIUM |
| 792b | CAMINIBACTER MEDIATLANTICUS MEDIUM |
| 792a | THERMOVIBRIO AMMONIFICANS MEDIUM |
| 1028 | THERMINCOLA MEDIUM (CO) |
| 1030 | SMITHELLA MEDIUM |
| 1040 | MARIDESULFOVIBRIO MEDIUM |
| 1041 | DESULFUROMUSA FERRIREDUCENS MEDIUM |
| 1042 | VULCANIBACILLUS MEDIUM |
| 1074 | DESULFOGLAEBA MEDIUM |
| 1083 | ACIDULIPROFUNDUM MEDIUM |
| 1092 | THERMOSULFIDIBACTER MEDIUM |
| 1098 | ARCHAEOGLOBUS INFECTUS MEDIUM |
| 1115 | CONGREGIBACTER (SYPHC) MEDIUM |
| 1188 | SPIROCHAETA CELLOBIOSIPHILA MEDIUM |
| 1188b | MARISPIROCHAETA MEDIUM |
| 1267b | GALLIONELLA (ES) MEDIUM |
| 1267a | MARIPROFUNDUS (ES) MEDIUM |
| 1459 | THERMOHALOBACTER MEDIUM |
| 1475 | METALLIBACTERIUM MEDIUM |
| 1526b | KIRITIMATIELLA (TYG) MEDIUM |
| 1665 | DESULFAMPLUS MEDIUM |