1459
1459 .
THERMOHALOBACTER MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1459 #
ml
Equipment needed: Hungate tubes
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| K2HPO4 | 0.3 | g | 0.3 | 1.722 | |
| KH2PO4 | 0.3 | g | 0.3 | 2.205 | |
| NH4Cl | 0.1 | g | 0.1 | 1.87 | |
| MgCl2 x 6 H2O | 2.0 | g | 2 | 9.838 | |
| CaCl2 x 2 H2O | 0.2 | g | 0.2 | 1.36 | |
| NaCl | 100.0 | g | 100 | 1711.16 | |
| KCl | 4.0 | g | 4 | 53.655 | |
| Na-acetate | 0.5 | g | 0.5 | 6.095 | |
|
Yeast extract
(OXOID) |
1.0 | g | 1 | - | |
|
Trypticase peptone
(BD BBL) |
1.0 | g | 1 | - | |
| Wolfe's mineral elixir | 1.0 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | - | |
| NaHCO3 | 2.5 | g | 2.5 | 29.759 | |
| D-Glucose | 10.0 | g | 10 | 55.506 | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
| Na2S x 9 H2O | 0.5 | g | 0.5 | 2.082 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients except bicarbonate, glucose, cysteine and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 7.0 - 7.2, if necessary. | |||||
Solution occurs in 21
other media (first defined in Medium 792).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MgSO4 x 7 H2O | 30.00 | g | 30 | 121.716 | |
| MnSO4 x H2O | 5.00 | g | 5 | 29.583 | |
| NaCl | 10.00 | g | 10 | 171.116 | |
| FeSO4 x 7 H2O | 1.00 | g | 1 | 3.597 | |
| CoCl2 x 6 H2O | 1.80 | g | 1.8 | 13.863 | |
| CaCl2 x 2 H2O | 1.00 | g | 1 | 6.802 | |
| ZnSO4 x 7 H2O | 1.80 | g | 1.8 | 6.26 | |
| CuSO4 x 5 H2O | 0.10 | g | 0.1 | 0.401 | |
| AlK(SO4)2 x 12 H2O | 0.18 | g | 0.18 | 0.379 | |
| H3BO3 | 0.10 | g | 0.1 | 1.617 | |
| Na2MoO4 x 2 H2O | 0.10 | g | 0.1 | 0.413 | |
| (NH4)2Ni(SO4)2 x 6 H2O | 2.80 | g | 2.8 | 7.089 | |
| Na2WO4 x 2 H2O | 0.10 | g | 0.1 | 0.303 | |
| Na2SeO4 | 0.10 | g | 0.1 | 0.529 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts. | |||||
Metadata
| Last modified: | 04.05.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 - 7.2 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 80 % N2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 2 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| D-Glucose | 10 | 55.506 |
| KCl | 4 | 53.655 |
| NaHCO3 | 2.5 | 29.759 |
| MgCl2 x 6 H2O | 2 | 9.838 |
| Yeast extract | 1 | - |
| Show more | ||
