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DSMZ 1098 . ARCHAEOGLOBUS INFECTUS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Sea Salt
(SIGMA)
35.00 g 34.93 -
NH4Cl 0.10 g 0.1 1.866
KH2PO4 0.05 g 0.05 0.367
Na-acetate 1.60 g 1.597 19.465
Yeast extract 0.10 g 0.1 -
Wolfe's mineral elixir 1.00 ml - -
Sodium resazurin
(0.1% w/v)
0.50 ml 5.0e-4 0.002
NaHCO3 2.00 g 1.996 23.76
2-Mercaptoethanesulfonic acid
(coenzyme M)
0.14 g 0.14 0.983
Seven vitamins solution 1.00 ml - -
Na2S2O3 x 5 H2O 2.50 g 2.495 10.053
Na2S x 9 H2O 0.50 g 0.499 2.078
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except bicarbonate, coenzyme M, vitamins, thiosulfate and sulfide), then sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 minto make it anoxic. Dispense medium under same gas atmosphere into anoxic serum vials (e.g., Balch-type tubes) and autoclave. Add coenzyme M, vitamins, thiosulfate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. The vitamins solution should be sterilized by filtration. Adjust pH of the complete medium to 6.5. After inoculation pressurize culture vessels to 1.5 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.
Solution occurs in 21 other media (first defined in Medium 792).
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 30.00 g 30 121.716
MnSO4 x H2O 5.00 g 5 29.583
NaCl 10.00 g 10 171.116
FeSO4 x 7 H2O 1.00 g 1 3.597
CoCl2 x 6 H2O 1.80 g 1.8 13.863
CaCl2 x 2 H2O 1.00 g 1 6.802
ZnSO4 x 7 H2O 1.80 g 1.8 6.26
CuSO4 x 5 H2O 0.10 g 0.1 0.401
AlK(SO4)2 x 12 H2O 0.18 g 0.18 0.379
H3BO3 0.10 g 0.1 1.617
Na2MoO4 x 2 H2O 0.10 g 0.1 0.413
(NH4)2Ni(SO4)2 x 6 H2O 2.80 g 2.8 7.089
Na2WO4 x 2 H2O 0.10 g 0.1 0.303
Na2SeO4 0.10 g 0.1 0.529
Distilled water 1000.00 ml - -
1 First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts.
Solution occurs in 77 other media (first defined in Medium 503).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 100 mg 0.1 0.074
p-Aminobenzoic acid 80 mg 0.08 0.583
D-(+)-biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
Calcium pantothenate 100 mg 0.1 0.21
Pyridoxine hydrochloride 300 mg 0.3 1.459
Thiamine-HCl x 2 H2O 200 mg 0.2 0.536
Distilled water 1000 ml - -

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 6.5
Equipment needed: Autoclave, Filter, Gassing station

Gas composition

  • 20 % CO2
  • 80 % H2

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sea Salt 34.93 -
Na2S2O3 x 5 H2O 2.495 10.053
NaHCO3 1.996 23.76
Na-acetate 1.597 19.465
Na2S x 9 H2O 0.499 2.078
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain

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