1665
1665 .
DESULFAMPLUS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1665 #
ml
Equipment needed: Autoclave
Hungate tubes
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 20.0 | g | 19.704 | 337.174 | |
| MgCl2 x 6 H2O | 6.0 | g | 5.911 | 29.077 | |
| Na2SO4 | 3.0 | g | 2.956 | 20.809 | |
| CaCl2 x 2 H2O | 0.2 | g | 0.197 | 1.34 | |
| KCl | 0.5 | g | 0.493 | 6.608 | |
| NH4Cl | 0.3 | g | 0.296 | 5.526 | |
| Wolfe's mineral elixir | 2.0 | ml | - | - | |
|
HEPES
(SIGMA) |
2.4 | g | 2.365 | 9.923 | |
| Na2-fumarate | 1.0 | g | 0.985 | 6.156 | |
|
Yeast extract
(OXOID) |
0.2 | g | 0.197 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 4.9e-4 | 0.002 | |
|
K-phosphate buffer
(0.5 M, pH 7.0) |
2.0 | ml | - | 1 | |
| NaHCO3 | 1.0 | g | 0.985 | 11.728 | |
|
FeCl2 x 4 H2O
(0.2% w/v in 0.02 N HCl) |
10.0 | ml | 0.02 | 0.099 | |
| Wolin's vitamin solution (10x) | 1.0 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.493 | 2.805 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients (except phosphate buffer, bicarbonate, ferrous chloride, vitamins and cysteine), adjust pH to 7.2 with NaOH and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add phosphate buffer, ferrous chloride, vitamins and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere and bicarbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins should be sterilized by filtration. Adjust pH of the complete medium to 7.2 - 7.5 , if necessary. | |||||
Solution occurs in 21
other media (first defined in Medium 792).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MgSO4 x 7 H2O | 30.00 | g | 30 | 121.716 | |
| MnSO4 x H2O | 5.00 | g | 5 | 29.583 | |
| NaCl | 10.00 | g | 10 | 171.116 | |
| FeSO4 x 7 H2O | 1.00 | g | 1 | 3.597 | |
| CoCl2 x 6 H2O | 1.80 | g | 1.8 | 13.863 | |
| CaCl2 x 2 H2O | 1.00 | g | 1 | 6.802 | |
| ZnSO4 x 7 H2O | 1.80 | g | 1.8 | 6.26 | |
| CuSO4 x 5 H2O | 0.10 | g | 0.1 | 0.401 | |
| AlK(SO4)2 x 12 H2O | 0.18 | g | 0.18 | 0.379 | |
| H3BO3 | 0.10 | g | 0.1 | 1.617 | |
| Na2MoO4 x 2 H2O | 0.10 | g | 0.1 | 0.413 | |
| (NH4)2Ni(SO4)2 x 6 H2O | 2.80 | g | 2.8 | 7.089 | |
| Na2WO4 x 2 H2O | 0.10 | g | 0.1 | 0.303 | |
| Na2SeO4 | 0.10 | g | 0.1 | 0.529 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts. | |||||
Solution occurs in 305
other media (first defined in Medium 120).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 20 | mg | 0.02 | 0.082 |
| Folic acid | 20 | mg | 0.02 | 0.045 |
| Pyridoxine hydrochloride | 100 | mg | 0.1 | 0.486 |
| Thiamine HCl | 50 | mg | 0.05 | 0.148 |
| Riboflavin | 50 | mg | 0.05 | 0.133 |
| Nicotinic acid | 50 | mg | 0.05 | 0.406 |
| Calcium D-(+)-pantothenate | 50 | mg | 0.05 | 0.21 |
| Vitamin B12 | 1 | mg | 1.0e-3 | 7.4e-4 |
| p-Aminobenzoic acid | 50 | mg | 0.05 | 0.365 |
| (DL)-alpha-Lipoic acid | 50 | mg | 0.05 | 0.242 |
| Distilled water | 1000 | ml | - | - |
Metadata
| Last modified: | 12.05.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 - 7.5 |
| Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| MgCl2 x 6 H2O | 5.911 | 29.077 |
| Na2SO4 | 2.956 | 20.809 |
| HEPES | 2.365 | 9.923 |
| NaHCO3 | 0.985 | 11.728 |
| Na2-fumarate | 0.985 | 6.156 |
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