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DSMZ

DSMZ 1167 . NSY-MEDIUM FOR POLYNUCLEOBACTER

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Polynucleobacter acidiphobus DSM 21994

* Modification: Agar surface must be kept moist all the time

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 25 °C

Main sol. 1167 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nutrient broth 1.0 g 1 -
Soy peptone
(e.g. Bacto Soytone) 		1.0 g 1 -
Yeast extract 1.0 g 1 -
Tap water 1000.0 ml - -
1 The following inorganic solution may substitute for tap water:
MgSO4 x 7 H2O 75.0 mg 0.075 0.304
Ca(NO3)2 x 4 H2O 43.0 mg 0.043 0.182
NaHCO3 16.0 mg 0.016 0.19
KCl 5.0 mg 0.005 0.067
K2HPO4 x 3 H2O 3.7 mg 0.004 0.021
Distilled water 1000.0 ml - -
Trace element solution SL-4 0.1 ml - -
2 Adjust to pH 7.2.

Trace element solution SL-4 #

1000
Solution occurs in 23 other media (first defined in Medium 14).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 0.50 g 0.5 1.487
FeSO4 x 7 H2O 0.20 g 0.2 0.719
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
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Associated strains

All strains for this medium: 9
Strains with modifications: 1
Polynucleobacter acidiphobus DSM 21994

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Nutrient broth 1 -
Yeast extract 1 -
Soy peptone 1 -
MgSO4 x 7 H2O 0.075 0.304
Ca(NO3)2 x 4 H2O 0.043 0.182
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Cultivation metadata from 9 strains BacDive

phyla 4 11 strains
nutrition chemoorganotroph 3 strains
nutrition chemoorganoheterotroph 2 strains
oxygen aerobe 5 strains
oxygen facultative anaerobe 1 strain
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