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1170. METHYLONATRUM MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.00 g 0.997 5.724
2 1.00 g 0.997 9.861
3 11.00 g 10.967 103.475
4 2.00 ml 1.575 49.163
5 18.00 g 17.946 307.074
6 0.16 g 0.16 2.982
7 0.24 g 0.239 0.971
8 24.00 mg 0.024 -
9 Trace element solution SL-6 1.00 ml - -
10 100.00 mg 0.1 0.063
11 1000.00 ml 995.221 55243.1
12 , might need strain adjustment 20.00 mM - -
Instructions:
  1. pH 10.0
  2. Prepare the medium without methanol, vitamin B12, MgSO4 x 7 H2O and NaS2O3 x 5 H2O in closed vessels with a medium to headspace ratio of 1:5 to 1:10. Add the methanol, MgSO4 x 7 H2O and NaS2O3 x 5 H2O from autoclaved stock solutions after sterilisation of the medium. Vitamin B12 is added from a filter sterilised stock solution to the sterile medium. Incubate the strains with shaking at 150 rpm. Growth is faster in the presence of 20 mM sodium acetate instead of methanol.
Solution occurs in 38 other media (first defined in medium 1142 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.10 g 0.1 0.348
2 0.03 g 0.03 0.152
3 0.30 g 0.3 4.852
4 0.20 g 0.2 0.841
5 0.01 g 0.01 0.059
6 0.02 g 0.02 0.084
7 0.03 g 0.03 0.124
8 1000.00 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 10.0
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 996.216 55298.3
NaCl 17.946 307.074
Na2CO3 10.967 103.475
Methanol 1.575 49.163
KNO3 0.997 9.861
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Cultivation metadata from 1 strains BacDive

halophily extreme 1 strain
halophily slightly 1 strain
pH 10 - 14 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
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