Name: METHYLONATRUM MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 1170 #

Equipment needed: Filter Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
K₂HPO₄	1.00	g	1	5.741
KNO₃	1.00	g	1	9.891
Na₂CO₃	11.00	g	11	103.785
Methanol	2.00	ml	1.584	49.435
NaCl	18.00	g	18	308.008
NH₄Cl	0.16	g	0.16	2.991
MgSO₄ x 7 H₂O	0.24	g	0.24	0.974
NaS₂O₃ x 5 H₂O	24.00	mg	0.024	-
Trace element solution SL-6	1.00	ml	-	-
Vitamin B₁₂	100.00	mg	0.1	0.074
Distilled water	1000.00	ml	-	-
1 pH 10.0
2 Prepare the medium without methanol, vitamin B₁₂, MgSO₄ x 7 H₂O and NaS₂O₃ x 5 H₂O in closed vessels with a medium to headspace ratio of 1:5 to 1:10. Add the methanol, MgSO₄ x 7 H₂O and NaS₂O₃ x 5 H₂O from autoclaved stock solutions after sterilisation of the medium. Vitamin B₁₂ is added from a filter sterilised stock solution to the sterile medium. Incubate the strains with shaking at 150 rpm. Growth is faster in the presence of 20 mM sodium acetate instead of methanol.

Trace element solution SL-6 #

1000

Solution occurs in 55 other media (first defined in Medium 27).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
ZnSO₄ x 7 H₂O	0.10	g	0.1	0.348
MnCl₂ x 4 H₂O	0.03	g	0.03	0.152
H₃BO₃	0.30	g	0.3	4.851
CoCl₂ x 6 H₂O	0.20	g	0.2	1.54
CuCl₂ x 2 H₂O	0.01	g	0.01	0.059
NiCl₂ x 6 H₂O	0.02	g	0.02	0.154
Na₂MoO₄ x 2 H₂O	0.03	g	0.03	0.124
Distilled water	1000.00	ml	-	-

All strains for this medium:

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